A γ-tocopherol-rich mixture of tocopherols inhibits colon inflammation and carcinogenesis in azoxymethane and dextran sulfate sodium-treated mice

Jihyeung Ju, Xingpei Hao, Mao Jung Lee, Joshua D. Lambert, Gang Lu, Hang Xiao, Harold L. Newmark, Chung S. Yang

Research output: Contribution to journalArticle

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Abstract

We investigated the effects of a γ-tocopherol-rich mixture of tocopherols (γ-TmT, containing 57% γ-T, 24% δ-T, and 13% α-T) on colon carcinogenesis in azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice. In experiment 1,6-week-old male CF-1 mice were given a dose of AOM (10 mg/kg body weight, i.p.), and 1 week later, 1.5% DSS in drinking water for 1 week. The mice were maintained on either a γ-TmT (0.3%)-enriched or a standard AIN93M diet, starting 1 week before the AOM injection, until the termination of experiment. In the AOM/DSS-treated mice, dietary γ-TmT treatment resulted in a significantly lower colon inflammation index (52% of the control) on day 7 and number of colon adeno-mas (9% of the control) on week 7. γ-TmT treatment also resulted in higher apoptotic index in adenomas, lower prostaglandin E2, leukotriene B4, and nitrotyrosine levels in the colon, and lower prostaglandin E2, leukotriene B4, and 8-isoprostane levels in the plasma on week 7. Some of the decreases were observed even on day 7. In experiment 2 with AOM/DSS-treated mice sacrificed on week 21, dietary 0.17% or 0.3% γ-TmT treatment, starting 1 week before the AOM injection, significantly inhibited adenocarcinoma and adenoma formation in the colon (to 17-33% of the control). Dietary 0.3% γ-TmT that was initiated after DSS treatment also exhibited a similar inhibitory activity. The present study showed that γ-TmT effectively inhibited colon carcinogenesis in AOM/DSS-treated mice, and the inhibition may be due to the apoptosis-inducing, anti-inflammatory, antioxidative, and reactive nitrogen species-trapping activities of tocopherols.

Original languageEnglish (US)
Pages (from-to)143-152
Number of pages10
JournalCancer Prevention Research
Volume2
Issue number2
DOIs
StatePublished - Feb 1 2009

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Azoxymethane
Dextran Sulfate
Tocopherols
Colon
Carcinogenesis
Inflammation
8-epi-prostaglandin F2alpha
Leukotriene B4
Dinoprostone
Adenoma
Reactive Nitrogen Species
Injections
Therapeutics
Drinking Water
Adenocarcinoma
Anti-Inflammatory Agents
Body Weight
Apoptosis
Diet

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Ju, Jihyeung ; Hao, Xingpei ; Lee, Mao Jung ; Lambert, Joshua D. ; Lu, Gang ; Xiao, Hang ; Newmark, Harold L. ; Yang, Chung S. / A γ-tocopherol-rich mixture of tocopherols inhibits colon inflammation and carcinogenesis in azoxymethane and dextran sulfate sodium-treated mice. In: Cancer Prevention Research. 2009 ; Vol. 2, No. 2. pp. 143-152.
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title = "A γ-tocopherol-rich mixture of tocopherols inhibits colon inflammation and carcinogenesis in azoxymethane and dextran sulfate sodium-treated mice",
abstract = "We investigated the effects of a γ-tocopherol-rich mixture of tocopherols (γ-TmT, containing 57{\%} γ-T, 24{\%} δ-T, and 13{\%} α-T) on colon carcinogenesis in azoxymethane (AOM)/dextran sulfate sodium (DSS)-treated mice. In experiment 1,6-week-old male CF-1 mice were given a dose of AOM (10 mg/kg body weight, i.p.), and 1 week later, 1.5{\%} DSS in drinking water for 1 week. The mice were maintained on either a γ-TmT (0.3{\%})-enriched or a standard AIN93M diet, starting 1 week before the AOM injection, until the termination of experiment. In the AOM/DSS-treated mice, dietary γ-TmT treatment resulted in a significantly lower colon inflammation index (52{\%} of the control) on day 7 and number of colon adeno-mas (9{\%} of the control) on week 7. γ-TmT treatment also resulted in higher apoptotic index in adenomas, lower prostaglandin E2, leukotriene B4, and nitrotyrosine levels in the colon, and lower prostaglandin E2, leukotriene B4, and 8-isoprostane levels in the plasma on week 7. Some of the decreases were observed even on day 7. In experiment 2 with AOM/DSS-treated mice sacrificed on week 21, dietary 0.17{\%} or 0.3{\%} γ-TmT treatment, starting 1 week before the AOM injection, significantly inhibited adenocarcinoma and adenoma formation in the colon (to 17-33{\%} of the control). Dietary 0.3{\%} γ-TmT that was initiated after DSS treatment also exhibited a similar inhibitory activity. The present study showed that γ-TmT effectively inhibited colon carcinogenesis in AOM/DSS-treated mice, and the inhibition may be due to the apoptosis-inducing, anti-inflammatory, antioxidative, and reactive nitrogen species-trapping activities of tocopherols.",
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A γ-tocopherol-rich mixture of tocopherols inhibits colon inflammation and carcinogenesis in azoxymethane and dextran sulfate sodium-treated mice. / Ju, Jihyeung; Hao, Xingpei; Lee, Mao Jung; Lambert, Joshua D.; Lu, Gang; Xiao, Hang; Newmark, Harold L.; Yang, Chung S.

In: Cancer Prevention Research, Vol. 2, No. 2, 01.02.2009, p. 143-152.

Research output: Contribution to journalArticle

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T1 - A γ-tocopherol-rich mixture of tocopherols inhibits colon inflammation and carcinogenesis in azoxymethane and dextran sulfate sodium-treated mice

AU - Ju, Jihyeung

AU - Hao, Xingpei

AU - Lee, Mao Jung

AU - Lambert, Joshua D.

AU - Lu, Gang

AU - Xiao, Hang

AU - Newmark, Harold L.

AU - Yang, Chung S.

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