A 2.8 Å Fe-Fe separation in the Fe2III/IV intermediate, X, from Escherichia coli ribonucleotide reductase

Laura M.K. Dassama, Alexey Silakov, Courtney M. Krest, Julio C. Calixto, Carsten Krebs, J. Martin Bollinger, Michael T. Green

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25 Scopus citations

Abstract

A class Ia ribonucleotide reductase (RNR) employs a μ-oxo-Fe 2III/III/tyrosyl radical cofactor in its β subunit to oxidize a cysteine residue ∼35 Å away in its α subunit; the resultant cysteine radical initiates substrate reduction. During self-assembly of the Escherichia coli RNR-β cofactor, reaction of the protein's Fe 2II/II complex with O2 results in accumulation of an Fe2III/IV cluster, termed X, which oxidizes the adjacent tyrosine (Y122) to the radical (Y122 ) as the cluster is converted to the μ-oxo-Fe 2III/III product. As the first high-valent non-heme-iron enzyme complex to be identified and the key activating intermediate of class Ia RNRs, X has been the focus of intensive efforts to determine its structure. Initial characterization by extended X-ray absorption fine structure (EXAFS) spectroscopy yielded a Fe-Fe separation (dFe-Fe) of 2.5 Å, which was interpreted to imply the presence of three single-atom bridges (O 2-, HO-, and/or μ-1,1-carboxylates). This short distance has been irreconcilable with computational and synthetic models, which all have dFe-Fe ≥ 2.7 Å. To resolve this conundrum, we revisited the EXAFS characterization of X. Assuming that samples containing increased concentrations of the intermediate would yield EXAFS data of improved quality, we applied our recently developed method of generating O2 in situ from chlorite using the enzyme chlorite dismutase to prepare X at ∼2.0 mM, more than 2.5 times the concentration realized in the previous EXAFS study. The measured dFe-Fe = 2.78 Å is fully consistent with computational models containing a (μ-oxo)2-Fe2 III/IV core. Correction of the dFe-Fe brings the experimental data and computational models into full conformity and informs analysis of the mechanism by which X generates Y122.

Original languageEnglish (US)
Pages (from-to)16758-16761
Number of pages4
JournalJournal of the American Chemical Society
Volume135
Issue number45
DOIs
StatePublished - Nov 13 2013

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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