A 2D surface morphology-composition gradient panel for protein-binding assays

Lai Wei, Erwin A. Vogler, Timothy M. Ritty, Akhlesh Lakhtakia

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We designed a two-dimensional (2D) morphology-composition gradient panel, which has a gradient in surface morphology at constant chemical composition along its length and a gradient in surface chemical composition without change of surface morphology in the orthogonal direction. The panels, which exhibit a 2D gradient in surface wettability, were fabricated by thermolysis of parylene-C followed by oxygen-plasma treatment for various times. As a demonstration study, binding of three different biotinylated serum proteins (B-IgG, B-A, and B-TF) to the 2D panel was investigated. The 2D gradient panels will facilitate development of optimized binding surfaces for various biotechnological applications.

Original languageEnglish (US)
Pages (from-to)1861-1866
Number of pages6
JournalMaterials Science and Engineering C
Volume31
Issue number8
DOIs
StatePublished - Dec 1 2011

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All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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