A 2D surface morphology-composition gradient panel for protein-binding assays

Lai Wei; Erwin A. Vogler; Timothy M. Ritty; Akhlesh Lakhtakia

doi:10.1016/j.msec.2011.09.001

A 2D surface morphology-composition gradient panel for protein-binding assays

Lai Wei, Erwin A. Vogler, Timothy M. Ritty, Akhlesh Lakhtakia

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

We designed a two-dimensional (2D) morphology-composition gradient panel, which has a gradient in surface morphology at constant chemical composition along its length and a gradient in surface chemical composition without change of surface morphology in the orthogonal direction. The panels, which exhibit a 2D gradient in surface wettability, were fabricated by thermolysis of parylene-C followed by oxygen-plasma treatment for various times. As a demonstration study, binding of three different biotinylated serum proteins (B-IgG, B-A, and B-TF) to the 2D panel was investigated. The 2D gradient panels will facilitate development of optimized binding surfaces for various biotechnological applications.

Original language	English (US)
Pages (from-to)	1861-1866
Number of pages	6
Journal	Materials Science and Engineering C
Volume	31
Issue number	8
DOIs	https://doi.org/10.1016/j.msec.2011.09.001
State	Published - Dec 1 2011

All Science Journal Classification (ASJC) codes

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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AU - Wei, Lai

AU - Vogler, Erwin A.

AU - Ritty, Timothy M.

AU - Lakhtakia, Akhlesh

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AB - We designed a two-dimensional (2D) morphology-composition gradient panel, which has a gradient in surface morphology at constant chemical composition along its length and a gradient in surface chemical composition without change of surface morphology in the orthogonal direction. The panels, which exhibit a 2D gradient in surface wettability, were fabricated by thermolysis of parylene-C followed by oxygen-plasma treatment for various times. As a demonstration study, binding of three different biotinylated serum proteins (B-IgG, B-A, and B-TF) to the 2D panel was investigated. The 2D gradient panels will facilitate development of optimized binding surfaces for various biotechnological applications.

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A 2D surface morphology-composition gradient panel for protein-binding assays

Abstract

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