TY - JOUR
T1 - A bidirectional rho-independent transcription terminator between the E. coli tonB gene and an opposing gene
AU - Postle, Kathleen
AU - Good, Rhonda F.
N1 - Funding Information:
We thank Kevin Bertrand, David Daniels, Janice Sharp, and Ron Wek for helpful discussions. We also thank Kevin Bertrand for critical reading of the manuscript, and Janice Sharp for plasmid DNA and reagents. This work was supported by a grant to K. R from the National Institutes of Health.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1985/6
Y1 - 1985/6
N2 - We identified an Escherichia coli gene, designated P14, that is adjacent to and in the opposite orientation to the tonB gene. The 36 base pair intercistronic region between tonB and P14 contains a novel rho-independent transcription terminator that functions bidirectionally, both in vivo and in vitro, to terminate tonB and P14 transcription. Transcription of tonB and P14 terminates at symmetrically equivalent nucleotides, such that the 3′ ends of tonB and P14 transcripts are complementary. The terminator is 70% efficient in both directions in vitro. Interestingly, relative rates of in vivo RNA synthesis, immediately prior to and following the terminator, appear to indicate that it is more efficient in the tonB direction (95%) than in the P14 direction (70%). We discuss the possibility that this gene arrangement has regulatory consequences for the expression of tonB.
AB - We identified an Escherichia coli gene, designated P14, that is adjacent to and in the opposite orientation to the tonB gene. The 36 base pair intercistronic region between tonB and P14 contains a novel rho-independent transcription terminator that functions bidirectionally, both in vivo and in vitro, to terminate tonB and P14 transcription. Transcription of tonB and P14 terminates at symmetrically equivalent nucleotides, such that the 3′ ends of tonB and P14 transcripts are complementary. The terminator is 70% efficient in both directions in vitro. Interestingly, relative rates of in vivo RNA synthesis, immediately prior to and following the terminator, appear to indicate that it is more efficient in the tonB direction (95%) than in the P14 direction (70%). We discuss the possibility that this gene arrangement has regulatory consequences for the expression of tonB.
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U2 - 10.1016/S0092-8674(85)80030-1
DO - 10.1016/S0092-8674(85)80030-1
M3 - Article
C2 - 2985285
AN - SCOPUS:0022351509
VL - 41
SP - 577
EP - 585
JO - Cell
JF - Cell
SN - 0092-8674
IS - 2
ER -