A block mixture model to map eQTLs for gene clustering and networking

Ningtao Wang, Kirk Gosik, Runze Li, Bruce Lindsay, Rongling Wu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To study how genes function in a cellular and physiological process, a general procedure is to classify gene expression profiles into categories based on their similarity and reconstruct a regulatory network for functional elements. However, this procedure has not been implemented with the genetic mechanisms that underlie the organization of gene clusters and networks, despite much effort made to map expression quantitative trait loci (eQTLs) that affect the expression of individual genes. Here we address this issue by developing a computational approach that integrates gene clustering and network reconstruction with genetic mapping into a unifying framework. The approach can not only identify specific eQTLs that control how genes are clustered and organized toward biological functions, but also enable the investigation of the biological mechanisms that individual eQTLs perturb in a signaling pathway. We applied the new approach to characterize the effects of eQTLs on the structure and organization of gene clusters in Caenorhabditis elegans. This study provides the first characterization, to our knowledge, of the effects of genetic variants on the regulatory network of gene expression. The approach developed can also facilitate the genetic dissection of other dynamic processes, including development, physiology and disease progression in any organisms.

Original languageEnglish (US)
Article number21193
JournalScientific reports
Volume6
DOIs
StatePublished - Feb 19 2016

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Quantitative Trait Loci
Cluster Analysis
Gene Regulatory Networks
Multigene Family
Physiological Phenomena
Gene Expression
Internal-External Control
Caenorhabditis elegans
Transcriptome
Disease Progression
Dissection
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "To study how genes function in a cellular and physiological process, a general procedure is to classify gene expression profiles into categories based on their similarity and reconstruct a regulatory network for functional elements. However, this procedure has not been implemented with the genetic mechanisms that underlie the organization of gene clusters and networks, despite much effort made to map expression quantitative trait loci (eQTLs) that affect the expression of individual genes. Here we address this issue by developing a computational approach that integrates gene clustering and network reconstruction with genetic mapping into a unifying framework. The approach can not only identify specific eQTLs that control how genes are clustered and organized toward biological functions, but also enable the investigation of the biological mechanisms that individual eQTLs perturb in a signaling pathway. We applied the new approach to characterize the effects of eQTLs on the structure and organization of gene clusters in Caenorhabditis elegans. This study provides the first characterization, to our knowledge, of the effects of genetic variants on the regulatory network of gene expression. The approach developed can also facilitate the genetic dissection of other dynamic processes, including development, physiology and disease progression in any organisms.",
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A block mixture model to map eQTLs for gene clustering and networking. / Wang, Ningtao; Gosik, Kirk; Li, Runze; Lindsay, Bruce; Wu, Rongling.

In: Scientific reports, Vol. 6, 21193, 19.02.2016.

Research output: Contribution to journalArticle

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