A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis

Sijie Hao, Gong Cheng, Yuan Wan, Yiqiu Xia, Donna M. Sosnoski, Andrea M. Mastro, Si Yang Zheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Bone is one of the preferred places for cancer metastasis, especially for breast, prostate, lung and melanoma cancers. In general, the prognosis for bone metastasis is abysmal with few treatment options available. Some colonies of cancer cells can reside in bone tissue as dormant cells for years before they become aggressive and grow into macrometastases. The interaction between cancer cells and bone tissue during this long period of time is currently not well understood. One technical barrier to study this in vitro is the lack of realistic bone tissue model suit for long-term study. Herein, we reported a bone-on-a-chip microdevice that can spontaneously form of a 3D, mineralized, collagenous bone tissue from an inoculum of isolated osteoblastic cell line without any artificial scaffold materials. Based on the principle of simultaneous-growth-dialysis, phenotypically mature osteoblastic tissue of up to 85 μm thickness containing heavily mineralized collagen fibers naturally formed in 720 hours without the aid of differentiation agents. Moreover, we have examined co-culture of metastatic human breast cancer cells MDA-MB-231 with osteoblastic tissues and observed important hallmarks of breast cancer colonization previously confirmed in vivo. With simple manipulation and ultra-low unit cost, the spontaneous 3D bone-on-a-chip shows promise as a physiologically-relevant model for the in vitro study of cancer bone metastasis.

Original languageEnglish (US)
Title of host publicationTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages2203-2206
Number of pages4
ISBN (Electronic)9781538627310
DOIs
StatePublished - Jul 26 2017
Event19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017 - Kaohsiung, Taiwan, Province of China
Duration: Jun 18 2017Jun 22 2017

Publication series

NameTRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017
CountryTaiwan, Province of China
CityKaohsiung
Period6/18/176/22/17

Fingerprint

metastasis
bones
Bone
cancer
chips
Tissue
Cells
breast
inoculum
multiple docking adapters
dialysis
Dialysis
prognosis
collagens
Cell culture
cultured cells
Collagen
Scaffolds
lungs
manipulators

All Science Journal Classification (ASJC) codes

  • Chemical Health and Safety
  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Hao, S., Cheng, G., Wan, Y., Xia, Y., Sosnoski, D. M., Mastro, A. M., & Zheng, S. Y. (2017). A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 2203-2206). [7994514] (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/TRANSDUCERS.2017.7994514
Hao, Sijie ; Cheng, Gong ; Wan, Yuan ; Xia, Yiqiu ; Sosnoski, Donna M. ; Mastro, Andrea M. ; Zheng, Si Yang. / A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis. TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 2203-2206 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).
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title = "A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis",
abstract = "Bone is one of the preferred places for cancer metastasis, especially for breast, prostate, lung and melanoma cancers. In general, the prognosis for bone metastasis is abysmal with few treatment options available. Some colonies of cancer cells can reside in bone tissue as dormant cells for years before they become aggressive and grow into macrometastases. The interaction between cancer cells and bone tissue during this long period of time is currently not well understood. One technical barrier to study this in vitro is the lack of realistic bone tissue model suit for long-term study. Herein, we reported a bone-on-a-chip microdevice that can spontaneously form of a 3D, mineralized, collagenous bone tissue from an inoculum of isolated osteoblastic cell line without any artificial scaffold materials. Based on the principle of simultaneous-growth-dialysis, phenotypically mature osteoblastic tissue of up to 85 μm thickness containing heavily mineralized collagen fibers naturally formed in 720 hours without the aid of differentiation agents. Moreover, we have examined co-culture of metastatic human breast cancer cells MDA-MB-231 with osteoblastic tissues and observed important hallmarks of breast cancer colonization previously confirmed in vivo. With simple manipulation and ultra-low unit cost, the spontaneous 3D bone-on-a-chip shows promise as a physiologically-relevant model for the in vitro study of cancer bone metastasis.",
author = "Sijie Hao and Gong Cheng and Yuan Wan and Yiqiu Xia and Sosnoski, {Donna M.} and Mastro, {Andrea M.} and Zheng, {Si Yang}",
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Hao, S, Cheng, G, Wan, Y, Xia, Y, Sosnoski, DM, Mastro, AM & Zheng, SY 2017, A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis. in TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems., 7994514, TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems, Institute of Electrical and Electronics Engineers Inc., pp. 2203-2206, 19th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2017, Kaohsiung, Taiwan, Province of China, 6/18/17. https://doi.org/10.1109/TRANSDUCERS.2017.7994514

A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis. / Hao, Sijie; Cheng, Gong; Wan, Yuan; Xia, Yiqiu; Sosnoski, Donna M.; Mastro, Andrea M.; Zheng, Si Yang.

TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc., 2017. p. 2203-2206 7994514 (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis

AU - Hao, Sijie

AU - Cheng, Gong

AU - Wan, Yuan

AU - Xia, Yiqiu

AU - Sosnoski, Donna M.

AU - Mastro, Andrea M.

AU - Zheng, Si Yang

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Y1 - 2017/7/26

N2 - Bone is one of the preferred places for cancer metastasis, especially for breast, prostate, lung and melanoma cancers. In general, the prognosis for bone metastasis is abysmal with few treatment options available. Some colonies of cancer cells can reside in bone tissue as dormant cells for years before they become aggressive and grow into macrometastases. The interaction between cancer cells and bone tissue during this long period of time is currently not well understood. One technical barrier to study this in vitro is the lack of realistic bone tissue model suit for long-term study. Herein, we reported a bone-on-a-chip microdevice that can spontaneously form of a 3D, mineralized, collagenous bone tissue from an inoculum of isolated osteoblastic cell line without any artificial scaffold materials. Based on the principle of simultaneous-growth-dialysis, phenotypically mature osteoblastic tissue of up to 85 μm thickness containing heavily mineralized collagen fibers naturally formed in 720 hours without the aid of differentiation agents. Moreover, we have examined co-culture of metastatic human breast cancer cells MDA-MB-231 with osteoblastic tissues and observed important hallmarks of breast cancer colonization previously confirmed in vivo. With simple manipulation and ultra-low unit cost, the spontaneous 3D bone-on-a-chip shows promise as a physiologically-relevant model for the in vitro study of cancer bone metastasis.

AB - Bone is one of the preferred places for cancer metastasis, especially for breast, prostate, lung and melanoma cancers. In general, the prognosis for bone metastasis is abysmal with few treatment options available. Some colonies of cancer cells can reside in bone tissue as dormant cells for years before they become aggressive and grow into macrometastases. The interaction between cancer cells and bone tissue during this long period of time is currently not well understood. One technical barrier to study this in vitro is the lack of realistic bone tissue model suit for long-term study. Herein, we reported a bone-on-a-chip microdevice that can spontaneously form of a 3D, mineralized, collagenous bone tissue from an inoculum of isolated osteoblastic cell line without any artificial scaffold materials. Based on the principle of simultaneous-growth-dialysis, phenotypically mature osteoblastic tissue of up to 85 μm thickness containing heavily mineralized collagen fibers naturally formed in 720 hours without the aid of differentiation agents. Moreover, we have examined co-culture of metastatic human breast cancer cells MDA-MB-231 with osteoblastic tissues and observed important hallmarks of breast cancer colonization previously confirmed in vivo. With simple manipulation and ultra-low unit cost, the spontaneous 3D bone-on-a-chip shows promise as a physiologically-relevant model for the in vitro study of cancer bone metastasis.

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M3 - Conference contribution

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Hao S, Cheng G, Wan Y, Xia Y, Sosnoski DM, Mastro AM et al. A bone-on-a-chip microdevice for long-term spontaneous 3D bone tissue formation and cancer bone metastasis. In TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems. Institute of Electrical and Electronics Engineers Inc. 2017. p. 2203-2206. 7994514. (TRANSDUCERS 2017 - 19th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/TRANSDUCERS.2017.7994514