A candidate pheromone receptor and two odorant receptors of the hawkmoth Manduca sexta

Harland M. Patch, Rodrigo A. Velarde, Kimberly K.O. Walden, Hugh M. Robertson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

In this study, we cloned and characterized three Manduca sexta odorant receptors (ORs). One receptor is a putative pheromone receptor expressed exclusively in a cell associated with male-specific type-I trichoid sensilla. We describe the results of real-time PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) experiments that show MsextaOR1 is expressed only in male antennae. In situ hybridization labels a single cell associated with type-1 trichoid sensilla, which houses two neurons that have been previously determined to respond to the major components of the pheromone blend. The second receptor, MsextaOR2, was discovered using degenerate primers designed to conserved motifs of a unique group ORs that share as much as 88% identity. Comparison of RT-PCR, qRT-PCR, and in situ hybridization results with those of ORs in the Drosophila melanogaster Or83b subfamily shows a strong sequence and expression pattern similarity. The third receptor, MsextaOR3, was found by 5′-end sequencing of a normalized and subtracted cDNA library from male M. sexta antennae. RT-PCR and qRT-PCR show that this receptor is expressed only in male and female antennae. These are the first ORs, including a putative pheromone receptor, to be described from M. sexta.

Original languageEnglish (US)
Pages (from-to)305-316
Number of pages12
JournalChemical senses
Volume34
Issue number4
DOIs
StatePublished - May 13 2009

Fingerprint

Pheromone Receptors
Odorant Receptors
Manduca
Real-Time Polymerase Chain Reaction
Sensilla
In Situ Hybridization
Pheromones
Drosophila melanogaster
Gene Library
Neurons

All Science Journal Classification (ASJC) codes

  • Physiology
  • Sensory Systems
  • Physiology (medical)
  • Behavioral Neuroscience

Cite this

Patch, Harland M. ; Velarde, Rodrigo A. ; Walden, Kimberly K.O. ; Robertson, Hugh M. / A candidate pheromone receptor and two odorant receptors of the hawkmoth Manduca sexta. In: Chemical senses. 2009 ; Vol. 34, No. 4. pp. 305-316.
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A candidate pheromone receptor and two odorant receptors of the hawkmoth Manduca sexta. / Patch, Harland M.; Velarde, Rodrigo A.; Walden, Kimberly K.O.; Robertson, Hugh M.

In: Chemical senses, Vol. 34, No. 4, 13.05.2009, p. 305-316.

Research output: Contribution to journalArticle

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