A carbon nanotube integrated microfluidic device for blood plasma extraction

Yin Ting Yeh, Zhong Lin, Si Yang Zheng, Mauricio Terrones

Research output: Contribution to journalArticle

Abstract

Blood is a complex fluid consisting of cells and plasma. Plasma contains key biomarkers essential for disease diagnosis and therapeutic monitoring. Thus, by separating plasma from the blood, it is possible to analyze these biomarkers. Conventional methods for plasma extraction involve bulky equipment, and miniaturization constitutes a key step to develop portable devices for plasma extraction. Here, we integrated nanomaterial synthesis with microfabrication, and built a microfluidic device. In particular, we designed a double-spiral channel able to perform cross-flow filtration. This channel was constructed by growing aligned carbon nanotubes (CNTs) with average inter-tubular distances of ~80 nm, which resulted in porosity values of ~93%. During blood extraction, these aligned CNTs allow smaller molecules (e.g., proteins) to pass through the channel wall, while larger molecules (e.g., cells) get blocked. Our results show that our device effectively separates plasma from blood, by trapping blood cells. We successfully recovered albumin -the most abundant protein inside plasma- with an efficiency of ~80%. This work constitutes the first report on integrating biocompatible nitrogen-doped CNT (CNxCNT) arrays to extract plasma from human blood, thus widening the bio-applications of CNTs.

Original languageEnglish (US)
Article number13623
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Lab-On-A-Chip Devices
Carbon Nanotubes
Equipment and Supplies
Biomarkers
Microtechnology
Miniaturization
Nanostructures
Porosity
Plasma Cells
Blood Proteins
Albumins
Blood Cells
Nitrogen

All Science Journal Classification (ASJC) codes

  • General

Cite this

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title = "A carbon nanotube integrated microfluidic device for blood plasma extraction",
abstract = "Blood is a complex fluid consisting of cells and plasma. Plasma contains key biomarkers essential for disease diagnosis and therapeutic monitoring. Thus, by separating plasma from the blood, it is possible to analyze these biomarkers. Conventional methods for plasma extraction involve bulky equipment, and miniaturization constitutes a key step to develop portable devices for plasma extraction. Here, we integrated nanomaterial synthesis with microfabrication, and built a microfluidic device. In particular, we designed a double-spiral channel able to perform cross-flow filtration. This channel was constructed by growing aligned carbon nanotubes (CNTs) with average inter-tubular distances of ~80 nm, which resulted in porosity values of ~93{\%}. During blood extraction, these aligned CNTs allow smaller molecules (e.g., proteins) to pass through the channel wall, while larger molecules (e.g., cells) get blocked. Our results show that our device effectively separates plasma from blood, by trapping blood cells. We successfully recovered albumin -the most abundant protein inside plasma- with an efficiency of ~80{\%}. This work constitutes the first report on integrating biocompatible nitrogen-doped CNT (CNxCNT) arrays to extract plasma from human blood, thus widening the bio-applications of CNTs.",
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A carbon nanotube integrated microfluidic device for blood plasma extraction. / Yeh, Yin Ting; Lin, Zhong; Zheng, Si Yang; Terrones, Mauricio.

In: Scientific reports, Vol. 8, No. 1, 13623, 01.12.2018.

Research output: Contribution to journalArticle

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