A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina

Martha Constantine-Paton, Andrew S. Blum, Rosalia Mendez-Otero, Colin Barnstable

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Brain topography may have its earliest expression as spatial gradients of molecules controlling the deposition of neurones and neuronal processes 1,2. In the vertebrate visual system there is evidence that the stereotyped alignment of central retinal projections relies on an initial spatially organized distribution of molecules in both the retina and its central target nuclei3-;6. We used an immunological approach to look for molecules that are so organized and produced a monoclonal antibody (JONES) which shows a pronounced dorsal to ventral gradient of binding in the rat retina throughout the period when retinal ganglion cell axons are forming topographically organized projections within the central nervous system (CNS). Binding is present throughout the radial thickness of the retinal epithelium in regions where post-mitotic neurones are generated but is not associated with any consistent histological characteristic of the tissue. The antibody was shown to bind on the cell surface of freshly dissociated retinal cells, and dorsal retinal quadrants were found in vitro to have nearly twice as much antigen as ventral retinal quadrants. Initial biochemical characterization of the target epitope reveals that it is a lipid present in chloroform/methanol extracts from perinatal retina and is sensitive to neuraminidase digestion.

Original languageEnglish (US)
Pages (from-to)459-462
Number of pages4
JournalNature
Volume324
Issue number6096
DOIs
StatePublished - Dec 1 1986

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Retina
Neurons
Retinal Ganglion Cells
Neuraminidase
Chloroform
Methanol
Axons
Vertebrates
Epitopes
Digestion
Epithelium
Central Nervous System
Monoclonal Antibodies
Lipids
Antigens
Antibodies
Brain
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • General

Cite this

Constantine-Paton, Martha ; Blum, Andrew S. ; Mendez-Otero, Rosalia ; Barnstable, Colin. / A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina. In: Nature. 1986 ; Vol. 324, No. 6096. pp. 459-462.
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A cell surface molecule distributed in a dorsoventral gradient in the perinatal rat retina. / Constantine-Paton, Martha; Blum, Andrew S.; Mendez-Otero, Rosalia; Barnstable, Colin.

In: Nature, Vol. 324, No. 6096, 01.12.1986, p. 459-462.

Research output: Contribution to journalArticle

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