A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC

Hridayabhiranjan Shukla, Gerald A. Gillespie, Rakesh Srivastava, Francis Collins, Michael J. Chorney

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

By the combination of cosmid cloning, chromosomal jumping, and pulsed-field gel electrophoresis (PFGE), we have fine-mapped the HLA-A subregion of the human major histocompatibility complex (MHC). Through the isolation of a class I jumping clone, the Qa-like HLA-G class I gene has been placed within 100 kb of HLA-H. The tight physical linkage of these class I genes has been further supported by hybridizing PFGE blots with locus-specific probes. It has been found that both of the above class I genes are linked to HLA-A, with HLA-H residing no more than 200 kb from the HLA-A gene. These data support the possible existence of a Qa-like subregion composed of nonclassical HLA class I genes within the human MHC linked telomerically to the HLA-A locus.

Original languageEnglish (US)
Pages (from-to)905-914
Number of pages10
JournalGenomics
Volume10
Issue number4
DOIs
StatePublished - Jan 1 1991

Fingerprint

HLA-G Antigens
MHC Class I Genes
HLA-A Antigens
Major Histocompatibility Complex
Clone Cells
Pulsed Field Gel Electrophoresis
Genes
Cosmids
Organism Cloning

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Shukla, Hridayabhiranjan ; Gillespie, Gerald A. ; Srivastava, Rakesh ; Collins, Francis ; Chorney, Michael J. / A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC. In: Genomics. 1991 ; Vol. 10, No. 4. pp. 905-914.
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A class I jumping clone places the HLA-G gene approximately 100 kilobases from HLA-H within the HLA-A subregion of the human MHC. / Shukla, Hridayabhiranjan; Gillespie, Gerald A.; Srivastava, Rakesh; Collins, Francis; Chorney, Michael J.

In: Genomics, Vol. 10, No. 4, 01.01.1991, p. 905-914.

Research output: Contribution to journalArticle

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