A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival

Ling Lin, Samantha White, Kebin Hu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Methods for the mechanistic investigations on renal fibrosis have long been concentrated on individual type of cells, such as fibroblasts and epithelial cells. However, in recent years, growing numbers of studies have been shifting toward the role of the intercellular interactions, such as communication between tubular epithelial cells and fibroblasts. Various co-culture models have been utilized in the studies of cell-cell communication and interaction. In this chapter, we describe an innovative co-culture model employing the porous membranes for spatially partitioning the cells while allowing direct crosstalk between fibroblasts and epithelial cells in an effort of mimicking in vivo environment.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages63-71
Number of pages9
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2346
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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