A comparative study of gangliosides from the brains of various species

Cara-Lynne Schengrund, O. W. Garrigan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A comparison is made of the sialic acid and hexose content of crude gangliosides from the brains of 13 species, including mammals, a bird, and amphibian, a reptile and fish. The sialic acid content is relatively constant from species to species. Gangliosides are less abundant in tissues other than the brain. Four species (cat, dog, pig and sheep) were selected for the determination of their major ganglioside subfractions. The ganglioside subfractions were isolated (after extraction and partition dialysis) using descending thin layer chromatography. The population of ganglioside molecules varied from species to species. From dog and sheep a mono-, a di- and a trisialoganglioside were obtained; from cat a mono-, a di- and two trisialogangliosides; and from pig a mono- and two disialogangliosides. Each ganglioside subfraction was found to contain glucose, galactose and galactosamine in the ratio of approximately 1:2:1. The fatty acid moieties consisted of more than 80% stearic acid with lesser amounts of arachidic, palmitic and behenic acid. Sphingosine analyses indicated ratios of sphingosine to icosisphingosine of 7:3 for the monosialo-, 1:1 for the disialo- and 3:7 for the trisialogangliosides.

Original languageEnglish (US)
Pages (from-to)488-495
Number of pages8
JournalLipids
Volume4
Issue number6
DOIs
StatePublished - Nov 1 1969

Fingerprint

gangliosides
Gangliosides
Brain
brain
sphingosine
Sphingosine
sialic acids
N-Acetylneuraminic Acid
Sheep
Cats
Swine
Dogs
cats
sheep
Thin layer chromatography
galactosamine
Galactosamine
swine
Mammals
Reptiles

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science

Cite this

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abstract = "A comparison is made of the sialic acid and hexose content of crude gangliosides from the brains of 13 species, including mammals, a bird, and amphibian, a reptile and fish. The sialic acid content is relatively constant from species to species. Gangliosides are less abundant in tissues other than the brain. Four species (cat, dog, pig and sheep) were selected for the determination of their major ganglioside subfractions. The ganglioside subfractions were isolated (after extraction and partition dialysis) using descending thin layer chromatography. The population of ganglioside molecules varied from species to species. From dog and sheep a mono-, a di- and a trisialoganglioside were obtained; from cat a mono-, a di- and two trisialogangliosides; and from pig a mono- and two disialogangliosides. Each ganglioside subfraction was found to contain glucose, galactose and galactosamine in the ratio of approximately 1:2:1. The fatty acid moieties consisted of more than 80{\%} stearic acid with lesser amounts of arachidic, palmitic and behenic acid. Sphingosine analyses indicated ratios of sphingosine to icosisphingosine of 7:3 for the monosialo-, 1:1 for the disialo- and 3:7 for the trisialogangliosides.",
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A comparative study of gangliosides from the brains of various species. / Schengrund, Cara-Lynne; Garrigan, O. W.

In: Lipids, Vol. 4, No. 6, 01.11.1969, p. 488-495.

Research output: Contribution to journalArticle

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