Two commercially available kits for determining blood ammonia were compared, an enzymatic method requiring a sample volume of 100μl and a nonenzymatic method requiring 1 ml of sample. Calibration curves for both kits were linear to 270 μmol/l, however correlation studies on plasma samples revealed a lack of agreement between methods (r = 0.825, Y = 0.76X + 51). Improved correlation occurs when portein interference is eliminated from the enzymatic procedure. Finally, the nonenzymatic method is modified to accommodate 100 μl of sample and compared to the original method.
All Science Journal Classification (ASJC) codes
- Clinical Biochemistry