A comparative study of micro-ammonia determinations in plasma using two different methods

Eric J. Sampson, Laurence M. Demers

Research output: Contribution to journalArticle

Abstract

Two commercially available kits for determining blood ammonia were compared, an enzymatic method requiring a sample volume of 100μl and a nonenzymatic method requiring 1 ml of sample. Calibration curves for both kits were linear to 270 μmol/l, however correlation studies on plasma samples revealed a lack of agreement between methods (r = 0.825, Y = 0.76X + 51). Improved correlation occurs when portein interference is eliminated from the enzymatic procedure. Finally, the nonenzymatic method is modified to accommodate 100 μl of sample and compared to the original method.

Original languageEnglish (US)
Pages (from-to)192-194
Number of pages3
JournalClinical Biochemistry
Volume9
Issue numberC
DOIs
StatePublished - 1976

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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