A comparison of blood factor XII autoactivation in buffer, protein cocktail, serum, and plasma solutions

A. Golas, C. H. Josh Yeh, H. Pitakjakpipop, Christopher Siedlecki, Erwin A. Vogler

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Activation of blood plasma coagulation in vitro by contact with material surfaces is demonstrably dependent on plasma-volume-to-activator-surface-area ratio. The only plausible explanation consistent with current understanding of coagulation-cascade biochemistry is that procoagulant stimulus arising from the activation complex of the intrinsic pathway is dependent on activator surface area. And yet, it is herein shown that activation of the blood zymogen factor XII (Hageman factor, FXII) dissolved in buffer, protein cocktail, heat-denatured serum, and FXI deficient plasma does not exhibit activator surface-area dependence. Instead, a highly-variable burst of procoagulant-enzyme yield is measured that exhibits no measurable kinetics, sensitivity to mixing, or solution-temperature dependence. Thus, FXII activation in both buffer and protein-containing solutions does not exhibit characteristics of a biochemical reaction but rather appears to be a " mechanochemical" reaction induced by FXII molecule interactions with hydrophilic activator particles that do not formally adsorb blood proteins from solution. Results of this study strongly suggest that activator surface-area dependence observed in contact activation of plasma coagulation does not solely arise at the FXII activation step of the intrinsic pathway.

Original languageEnglish (US)
Pages (from-to)607-620
Number of pages14
JournalBiomaterials
Volume34
Issue number3
DOIs
StatePublished - Jan 1 2013

Fingerprint

Factor XII
Blood Proteins
Buffers
Blood
Chemical activation
Proteins
Plasmas
Coagulation
Enzyme Precursors
Plasma Volume
Blood Coagulation
Hydrophobic and Hydrophilic Interactions
Biochemistry
Hot Temperature
Enzyme kinetics
Temperature
Enzymes
Serum
Molecules
Kinetics

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Golas, A. ; Josh Yeh, C. H. ; Pitakjakpipop, H. ; Siedlecki, Christopher ; Vogler, Erwin A. / A comparison of blood factor XII autoactivation in buffer, protein cocktail, serum, and plasma solutions. In: Biomaterials. 2013 ; Vol. 34, No. 3. pp. 607-620.
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A comparison of blood factor XII autoactivation in buffer, protein cocktail, serum, and plasma solutions. / Golas, A.; Josh Yeh, C. H.; Pitakjakpipop, H.; Siedlecki, Christopher; Vogler, Erwin A.

In: Biomaterials, Vol. 34, No. 3, 01.01.2013, p. 607-620.

Research output: Contribution to journalArticle

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