A comparison of cytoplasmic revertants to fertility from different CMS-S maize sources

I. D. Small, E. D. Earle, Loida J. Escote-Carlson, S. Gabay-Laughnan, J. R. Laughnan, C. J. Leaver

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The mitochondrial genome organizations of a number of independent culture-derived fertile CMS-S revertants with the nuclear genotype W182BN were compared to spontaneous field revertants with the genotypes WF9, M825/Oh07 and 38-11. Regions of the genome around sequences homologous to the terminal repeats of the linear S1 and S2 episomes characteristic of CMS-S mitochondria were used as hybridization probes on Southern blots of BamHI and SalI digested mitochondrial DNA. The results obtained suggest that the nuclear, not the cytoplasmic, genotype of the parent plant affects the type of novel mitochondrial DNA organization found in the revertant. The DNA reorganization during reversion from CMS-S in tissue culture appears to be similar to that observed in spontaneous revertants obtained during the normal plant life-cycle. Unlike the situation for reversion from CMS-T, no common DNA sequence or reading frame appeared to be lost or disrupted in revertants.

Original languageEnglish (US)
Pages (from-to)609-618
Number of pages10
JournalTheoretical and Applied Genetics
Volume76
Issue number4
DOIs
StatePublished - Oct 1 1988

Fingerprint

Zea mays
Fertility
Genotype
Mitochondrial DNA
corn
genotype
mitochondrial DNA
Reading Frames
terminal repeat sequences
Mitochondrial Genome
Terminal Repeat Sequences
Sequence Homology
Southern Blotting
Life Cycle Stages
sequence homology
Southern blotting
tissue culture
life cycle (organisms)
plasmids
Mitochondria

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Small, I. D. ; Earle, E. D. ; Escote-Carlson, Loida J. ; Gabay-Laughnan, S. ; Laughnan, J. R. ; Leaver, C. J. / A comparison of cytoplasmic revertants to fertility from different CMS-S maize sources. In: Theoretical and Applied Genetics. 1988 ; Vol. 76, No. 4. pp. 609-618.
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A comparison of cytoplasmic revertants to fertility from different CMS-S maize sources. / Small, I. D.; Earle, E. D.; Escote-Carlson, Loida J.; Gabay-Laughnan, S.; Laughnan, J. R.; Leaver, C. J.

In: Theoretical and Applied Genetics, Vol. 76, No. 4, 01.10.1988, p. 609-618.

Research output: Contribution to journalArticle

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AU - Small, I. D.

AU - Earle, E. D.

AU - Escote-Carlson, Loida J.

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AU - Laughnan, J. R.

AU - Leaver, C. J.

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