A comparison of susceptibility to Myxobolus cerebralis among strains of rainbow trout and steelhead in field and laboratory trials

C. L. Densmore, Vicki Suzette Blazer, D. D. Cartwright, W. B. Schill, J. H. Schachte, C. J. Petrie, M. V. Batur, T. B. Waldrop, A. Mack, P. S. Pooler

Research output: Contribution to journalArticle

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Abstract

Three strains of rainbow trout and steelhead Oncorhynchus mykiss were evaluated for the presence of whirling disease in field and laboratory trials. In the field exposures, fingerling Salmon River steelhead and Cayuga Lake and Randolph strains of rainbow trout were placed in wire cages in an earthen, stream-fed pond in New York State that was known to harbor Myxobolus cerebralis. Control fish were held at another hatchery that was free of whirling disease. In the controlled trials at the National Fish Health Research Laboratory, fingerling steelhead and Cayuga Lake and Mount Lassen rainbow trout were exposed to triactinomyxons at low (200 triactinomyxons/fish) or high (2,000 triactinomyxons/fish) levels for 2 h. Controls of each group were sham-exposed. Following an incubation period of 154 d for laboratory trials and 180 d for field trials, cranial tissue samples were taken for spore enumeration (field and laboratory trials) and histological analyses (laboratory only). Clinical signs of disease, including whirling behavior, blacktail, and skeletal deformities, were recorded for each fish in the laboratory trial at the terminal sampling. No clinical evidence of disease was noted among fish in the field trials. Clinical signs were noted among all strains in the laboratory trials at both exposure levels, and these signs were consistently greatest for the Mount Lassen strain. Whirling and skeletal deformities were more evident in the steelhead than in the Cayuga Lake rainbow trout; blacktail was more common in the Cayuga Lake fish. In both field and laboratory trials, spore counts were significantly higher for Cayuga Lake rainbow trout than in steelhead. In laboratory trials, moderate to marked cranial tissue lesions predominated in all three strains.

Original languageEnglish (US)
Pages (from-to)220-227
Number of pages8
JournalJournal of Aquatic Animal Health
Volume13
Issue number3
DOIs
StatePublished - Jan 1 2001

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Myxobolus cerebralis
rainbow
Oncorhynchus mykiss
whirling disease
fish
lakes
lake
fingerlings
field experimentation
spore
spores
laboratory
comparison
trial
fish health
wire
lesions (animal)
hatcheries
salmon
hatchery

All Science Journal Classification (ASJC) codes

  • Aquatic Science

Cite this

Densmore, C. L. ; Blazer, Vicki Suzette ; Cartwright, D. D. ; Schill, W. B. ; Schachte, J. H. ; Petrie, C. J. ; Batur, M. V. ; Waldrop, T. B. ; Mack, A. ; Pooler, P. S. / A comparison of susceptibility to Myxobolus cerebralis among strains of rainbow trout and steelhead in field and laboratory trials. In: Journal of Aquatic Animal Health. 2001 ; Vol. 13, No. 3. pp. 220-227.
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abstract = "Three strains of rainbow trout and steelhead Oncorhynchus mykiss were evaluated for the presence of whirling disease in field and laboratory trials. In the field exposures, fingerling Salmon River steelhead and Cayuga Lake and Randolph strains of rainbow trout were placed in wire cages in an earthen, stream-fed pond in New York State that was known to harbor Myxobolus cerebralis. Control fish were held at another hatchery that was free of whirling disease. In the controlled trials at the National Fish Health Research Laboratory, fingerling steelhead and Cayuga Lake and Mount Lassen rainbow trout were exposed to triactinomyxons at low (200 triactinomyxons/fish) or high (2,000 triactinomyxons/fish) levels for 2 h. Controls of each group were sham-exposed. Following an incubation period of 154 d for laboratory trials and 180 d for field trials, cranial tissue samples were taken for spore enumeration (field and laboratory trials) and histological analyses (laboratory only). Clinical signs of disease, including whirling behavior, blacktail, and skeletal deformities, were recorded for each fish in the laboratory trial at the terminal sampling. No clinical evidence of disease was noted among fish in the field trials. Clinical signs were noted among all strains in the laboratory trials at both exposure levels, and these signs were consistently greatest for the Mount Lassen strain. Whirling and skeletal deformities were more evident in the steelhead than in the Cayuga Lake rainbow trout; blacktail was more common in the Cayuga Lake fish. In both field and laboratory trials, spore counts were significantly higher for Cayuga Lake rainbow trout than in steelhead. In laboratory trials, moderate to marked cranial tissue lesions predominated in all three strains.",
author = "Densmore, {C. L.} and Blazer, {Vicki Suzette} and Cartwright, {D. D.} and Schill, {W. B.} and Schachte, {J. H.} and Petrie, {C. J.} and Batur, {M. V.} and Waldrop, {T. B.} and A. Mack and Pooler, {P. S.}",
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Densmore, CL, Blazer, VS, Cartwright, DD, Schill, WB, Schachte, JH, Petrie, CJ, Batur, MV, Waldrop, TB, Mack, A & Pooler, PS 2001, 'A comparison of susceptibility to Myxobolus cerebralis among strains of rainbow trout and steelhead in field and laboratory trials', Journal of Aquatic Animal Health, vol. 13, no. 3, pp. 220-227. https://doi.org/10.1577/1548-8667(2001)013<0220:ACOSTM>2.0.CO;2

A comparison of susceptibility to Myxobolus cerebralis among strains of rainbow trout and steelhead in field and laboratory trials. / Densmore, C. L.; Blazer, Vicki Suzette; Cartwright, D. D.; Schill, W. B.; Schachte, J. H.; Petrie, C. J.; Batur, M. V.; Waldrop, T. B.; Mack, A.; Pooler, P. S.

In: Journal of Aquatic Animal Health, Vol. 13, No. 3, 01.01.2001, p. 220-227.

Research output: Contribution to journalArticle

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T1 - A comparison of susceptibility to Myxobolus cerebralis among strains of rainbow trout and steelhead in field and laboratory trials

AU - Densmore, C. L.

AU - Blazer, Vicki Suzette

AU - Cartwright, D. D.

AU - Schill, W. B.

AU - Schachte, J. H.

AU - Petrie, C. J.

AU - Batur, M. V.

AU - Waldrop, T. B.

AU - Mack, A.

AU - Pooler, P. S.

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N2 - Three strains of rainbow trout and steelhead Oncorhynchus mykiss were evaluated for the presence of whirling disease in field and laboratory trials. In the field exposures, fingerling Salmon River steelhead and Cayuga Lake and Randolph strains of rainbow trout were placed in wire cages in an earthen, stream-fed pond in New York State that was known to harbor Myxobolus cerebralis. Control fish were held at another hatchery that was free of whirling disease. In the controlled trials at the National Fish Health Research Laboratory, fingerling steelhead and Cayuga Lake and Mount Lassen rainbow trout were exposed to triactinomyxons at low (200 triactinomyxons/fish) or high (2,000 triactinomyxons/fish) levels for 2 h. Controls of each group were sham-exposed. Following an incubation period of 154 d for laboratory trials and 180 d for field trials, cranial tissue samples were taken for spore enumeration (field and laboratory trials) and histological analyses (laboratory only). Clinical signs of disease, including whirling behavior, blacktail, and skeletal deformities, were recorded for each fish in the laboratory trial at the terminal sampling. No clinical evidence of disease was noted among fish in the field trials. Clinical signs were noted among all strains in the laboratory trials at both exposure levels, and these signs were consistently greatest for the Mount Lassen strain. Whirling and skeletal deformities were more evident in the steelhead than in the Cayuga Lake rainbow trout; blacktail was more common in the Cayuga Lake fish. In both field and laboratory trials, spore counts were significantly higher for Cayuga Lake rainbow trout than in steelhead. In laboratory trials, moderate to marked cranial tissue lesions predominated in all three strains.

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