A complex chromatin landscape revealed by patterns of nuclease sensitivity and histone modification within the mouse β-globin locus

Michael Bulger, Dirk Schübeler, M. A. Bender, Joan Hamilton, Catherine M. Farrell, Ross Cameron Hardison, Mark Groudine

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

In order to create an extended map of chromatin features within a mammalian multigene locus, we have determined the extent of nuclease sensitivity and the pattern of histone modifications associated with the mouse β-globin genes in adult erythroid tissue. We show that the nuclease-sensitive domain encompasses the β-globin genes along with several flanking olfactory receptor genes that are inactive in erythroid cells. We describe enhancer-blocking or boundary elements on either side of the locus that are bound in vivo by the transcription factor CTCF, but we found that they do not coincide with transitions in nuclease sensitivity flanking the locus or with patterns of histone modifications within it. In addition, histone hyperacetylation and dimethylation of histone H3 K4 are not uniform features of the nuclease-sensitive mouse β-globin domain but rather define distinct subdomains within it. Our results reveal a complex chromatin landscape for the active β-globin locus and illustrate the complexity of broad structural changes that accompany gene activation.

Original languageEnglish (US)
Pages (from-to)5234-5244
Number of pages11
JournalMolecular and cellular biology
Volume23
Issue number15
DOIs
StatePublished - Aug 1 2003

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Histone Code
Globins
Chromatin
Histones
Insulator Elements
Odorant Receptors
Genes
Erythroid Cells
Transcriptional Activation
Transcription Factors

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Bulger, Michael ; Schübeler, Dirk ; Bender, M. A. ; Hamilton, Joan ; Farrell, Catherine M. ; Hardison, Ross Cameron ; Groudine, Mark. / A complex chromatin landscape revealed by patterns of nuclease sensitivity and histone modification within the mouse β-globin locus. In: Molecular and cellular biology. 2003 ; Vol. 23, No. 15. pp. 5234-5244.
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A complex chromatin landscape revealed by patterns of nuclease sensitivity and histone modification within the mouse β-globin locus. / Bulger, Michael; Schübeler, Dirk; Bender, M. A.; Hamilton, Joan; Farrell, Catherine M.; Hardison, Ross Cameron; Groudine, Mark.

In: Molecular and cellular biology, Vol. 23, No. 15, 01.08.2003, p. 5234-5244.

Research output: Contribution to journalArticle

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