A computerized microspectrophotometer using fibre optics for transmission and detection of light

John Neely, William J. Townend, John W. Combs

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

A computerized microspectrophotometer was developed to provide rapid and accurate sequential measurements on cells, including nuclear DNA content, by fluorescence staining, photometric grain counting from autoradiographs, and nuclear size estimated by measurement aperture area. To alleviate bulk and vibrations caused by additional stepper motors, the excitation light and filters as well as the emission filters and photomultipliers were detached from the main microscope frame. The light was directed to and from the microscope through fibre optic bundles. This configuration provided an excellent light gathering faculty and made optical alignment easier. The machine provided highly accurate sequential measurements on a relatively large number of cells by static photometry. 1984 Blackwell Science Ltd

Original languageEnglish (US)
Pages (from-to)313-322
Number of pages10
JournalJournal of Microscopy
Volume133
Issue number3
DOIs
StatePublished - Jan 1 1984

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Histology

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