A contig map of the Mhc class I genomic region in the zebrafish reveals ancient synteny

Věra Michalová, Brent W. Murray, Holger Sültmann, Jan Klein

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

In contrast to the human and mouse Mhe, in which the clusters of class I and class II loci reside in close vicinity to one another, in the zebrafish, Danio rerio, they are found in different linkage groups. Chromosome walking using BAC (bacterial artificial chromosome) and PAC (P1 artificial chromosome) clones reveals the zebrafish class I region to occupy a segment of ~450 kb and to encompass at least 19 loci. These include three class l (Dare-UDA, -UEA, -UFA), five proteasome subunit β (PSMB8, -9A, -9C, -11, - 12), two TAPs (TAP2A, TAP2B), and one TAP binding protein (TAPBP). This arrangement contrasts with the arrangements found in human and mouse Mhc, in which the orthologues of the PSMB, TAP, and TAPBP loci reside within the class II region. In addition to this main zebrafish class I contig, a shorter contig of about 150 kb contains two additional class I (UBA, UCA) and at least five other loci. It probably represents a different haplotype of part of the class I region. The previously identified UAA gene shares an identical 5' part with UEA, but the two genes differ in their 3' parts. One of them is probably the result of an unequal crossing over. The described organization has implications for the persistence of syntenic relationships, coevolution of loci, and interpretation of the origin of the human/mouse Mhe organization.

Original languageEnglish (US)
Pages (from-to)5296-5305
Number of pages10
JournalJournal of Immunology
Volume164
Issue number10
DOIs
StatePublished - May 15 2000

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Synteny
Zebrafish
Carrier Proteins
Chromosome Walking
Artificial Chromosomes
Bacterial Artificial Chromosomes
Proteasome Endopeptidase Complex
Haplotypes
Genes
Clone Cells

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Michalová, Věra ; Murray, Brent W. ; Sültmann, Holger ; Klein, Jan. / A contig map of the Mhc class I genomic region in the zebrafish reveals ancient synteny. In: Journal of Immunology. 2000 ; Vol. 164, No. 10. pp. 5296-5305.
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A contig map of the Mhc class I genomic region in the zebrafish reveals ancient synteny. / Michalová, Věra; Murray, Brent W.; Sültmann, Holger; Klein, Jan.

In: Journal of Immunology, Vol. 164, No. 10, 15.05.2000, p. 5296-5305.

Research output: Contribution to journalArticle

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