A defined antigen skin test for the diagnosis of bovine tuberculosis

Sreenidhi Srinivasan, Gareth Jones, Maroudam Veerasami, Sabine Steinbach, Thomas Holder, Aboma Zewude, Abebe Fromsa, Gobena Ameni, Laurel Easterling, Douwe Bakker, Nicholas Juleff, Glen Gifford, R. G. Hewinson, H. Martin Vordermeier, Vivek Kapur

Research output: Contribution to journalArticle

Abstract

Bovine tuberculosis (bTB) is a major zoonotic disease of cattle that is endemic in much of the world, limiting livestock productivity and representing a global public health threat. Because the standard tuberculin skin test precludes implementation of Bacille Calmette-Guérin (BCG) vaccine–based control programs, we here developed and evaluated a novel peptide-based defined antigen skin test (DST) to diagnose bTB and to differentiate infected from vaccinated animals (DIVA). The results, in laboratory assays and in experimentally or naturally infected animals, demonstrate that the peptide-based DST provides DIVA capability and equal or superior performance over the extant standard tuberculin surveillance test. Together with the ease of chemical synthesis, quality control, and lower burden for regulatory approval compared with recombinant antigens, the results of our studies show that the DST considerably improves a century-old standard and enables the development and implementation of critically needed surveillance and vaccination programs to accelerate bTB control.

Original languageEnglish (US)
Article numbereaax4899
JournalScience Advances
Volume5
Issue number7
DOIs
StatePublished - Jul 17 2019

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tuberculosis
antigens
animals
surveillance
peptides
livestock
cattle
public health
quality control
productivity
synthesis

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)
  • General

Cite this

Srinivasan, S., Jones, G., Veerasami, M., Steinbach, S., Holder, T., Zewude, A., ... Kapur, V. (2019). A defined antigen skin test for the diagnosis of bovine tuberculosis. Science Advances, 5(7), [eaax4899]. https://doi.org/10.1126/sciadv.aax4899
Srinivasan, Sreenidhi ; Jones, Gareth ; Veerasami, Maroudam ; Steinbach, Sabine ; Holder, Thomas ; Zewude, Aboma ; Fromsa, Abebe ; Ameni, Gobena ; Easterling, Laurel ; Bakker, Douwe ; Juleff, Nicholas ; Gifford, Glen ; Hewinson, R. G. ; Martin Vordermeier, H. ; Kapur, Vivek. / A defined antigen skin test for the diagnosis of bovine tuberculosis. In: Science Advances. 2019 ; Vol. 5, No. 7.
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Srinivasan, S, Jones, G, Veerasami, M, Steinbach, S, Holder, T, Zewude, A, Fromsa, A, Ameni, G, Easterling, L, Bakker, D, Juleff, N, Gifford, G, Hewinson, RG, Martin Vordermeier, H & Kapur, V 2019, 'A defined antigen skin test for the diagnosis of bovine tuberculosis', Science Advances, vol. 5, no. 7, eaax4899. https://doi.org/10.1126/sciadv.aax4899

A defined antigen skin test for the diagnosis of bovine tuberculosis. / Srinivasan, Sreenidhi; Jones, Gareth; Veerasami, Maroudam; Steinbach, Sabine; Holder, Thomas; Zewude, Aboma; Fromsa, Abebe; Ameni, Gobena; Easterling, Laurel; Bakker, Douwe; Juleff, Nicholas; Gifford, Glen; Hewinson, R. G.; Martin Vordermeier, H.; Kapur, Vivek.

In: Science Advances, Vol. 5, No. 7, eaax4899, 17.07.2019.

Research output: Contribution to journalArticle

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Srinivasan S, Jones G, Veerasami M, Steinbach S, Holder T, Zewude A et al. A defined antigen skin test for the diagnosis of bovine tuberculosis. Science Advances. 2019 Jul 17;5(7). eaax4899. https://doi.org/10.1126/sciadv.aax4899