A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1

Weiguo Zhang, Yanming Wang, Jin Long, Jack Girton, Jørgen Johansen, Kristen M. Johansen

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Using a yeast two-hybrid screen we have identified a novel isoform of the lola locus, Lola zf5, that interacts with the chromosomal kinase JIL-1. We characterized the lola locus and provide evidence that it is a complex locus from which at least 17 different splice variants are likely to be generated. Fifteen of these each have a different zinc finger domain, whereas two are without. This potential for expression of multiple gene products suggests that they serve diverse functional roles in different developmental contexts. By Northern and Western blot analyses we demonstrate that the expression of Lola zf5 is developmentally regulated and that it is restricted to early embryogenesis. Immunocytochemical labeling with a Lola zf5-specific antibody of Drosophila embryos indicates that Lola zf5 is localized to nuclei. Furthermore, by creating double-mutant flies we show that a reduction of Lola protein levels resulting from mutations in the lola locus acts as a dominant modifier of a hypomorphic JIL-1 allele leading to an increase in embryonic viability. Thus, genetic interaction assays provide direct evidence that gene products from the lola locus function within the same pathway as the chromosomal kinase JIL-1.

Original languageEnglish (US)
Pages (from-to)11696-11704
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number13
DOIs
StatePublished - Mar 28 2003

Fingerprint

Zinc Fingers
Zinc
Phosphotransferases
Genes
Diptera
Northern Blotting
Yeast
Labeling
Drosophila
Embryonic Development
Assays
Protein Isoforms
Proteins
Embryonic Structures
Yeasts
Western Blotting
Alleles
Gene Expression
Mutation
Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Zhang, Weiguo ; Wang, Yanming ; Long, Jin ; Girton, Jack ; Johansen, Jørgen ; Johansen, Kristen M. / A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 13. pp. 11696-11704.
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A developmentally regulated splice variant from the complex lola locus encoding multiple different zinc finger domain proteins interacts with the chromosomal kinase JIL-1. / Zhang, Weiguo; Wang, Yanming; Long, Jin; Girton, Jack; Johansen, Jørgen; Johansen, Kristen M.

In: Journal of Biological Chemistry, Vol. 278, No. 13, 28.03.2003, p. 11696-11704.

Research output: Contribution to journalArticle

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AU - Johansen, Kristen M.

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