A diverse soil microbiome degrades more crude oil than specialized bacterial assemblages obtained in culture

Terrence H. Bell, Franck O.P. Stefani, Katrina Abram, Julie Champagne, Etienne Yergeau, Mohamed Hijri, Marc St-Arnaud

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Soil microbiome modification may alter system function, which may enhance processes like bioremediation. In this study, we filled microcosms with gamma-irradiated soil that was reinoculated with the initial soil or cultivated bacterial subsets obtained on regular media (REG-M) or media containing crude oil (CO-M). We allowed 8 weeks for microbiome stabilization, added crude oil and monoammonium phosphate, incubated the microcosms for another 6 weeks, and then measured the biodegradation of crude oil components, bacterial taxonomy, and functional gene composition. We hypothesized that the biodegradation of targeted crude oil components would be enhanced by limiting the microbial taxa competing for resources and by specifically selecting bacteria involved in crude oil biodegradation (i.e., CO-M). Postincubation, large differences in taxonomy and functional gene composition between the three microbiome types remained, indicating that purposeful soil microbiome structuring is feasible. Although phylum-level bacterial taxonomy was constrained, operational taxonomic unit composition varied between microbiome types. Contrary to our hypothesis, the biodegradation of C10 to C50 hydrocarbons was highest when the original microbiome was reinoculated, despite a higher relative abundance of alkane hydroxylase genes in the CO-M microbiomes and of carbon-processing genes in the REG-M microbiomes. Despite increases in the relative abundances of genes potentially linked to hydrocarbon processing in cultivated subsets of the microbiome, reinoculation of the initial microbiome led to maximum biodegradation.

Original languageEnglish (US)
Pages (from-to)5530-5541
Number of pages12
JournalApplied and environmental microbiology
Volume82
Issue number18
DOIs
StatePublished - Jan 1 2016

Fingerprint

Microbiota
Petroleum
crude oil
biodegradation
Soil
oils
gene
soil
microcosm
relative abundance
Carbon Monoxide
hydrocarbon
Genes
Hydrocarbons
taxonomy
genes
bioremediation
alkane
hydrocarbons
stabilization

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Bell, Terrence H. ; Stefani, Franck O.P. ; Abram, Katrina ; Champagne, Julie ; Yergeau, Etienne ; Hijri, Mohamed ; St-Arnaud, Marc. / A diverse soil microbiome degrades more crude oil than specialized bacterial assemblages obtained in culture. In: Applied and environmental microbiology. 2016 ; Vol. 82, No. 18. pp. 5530-5541.
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A diverse soil microbiome degrades more crude oil than specialized bacterial assemblages obtained in culture. / Bell, Terrence H.; Stefani, Franck O.P.; Abram, Katrina; Champagne, Julie; Yergeau, Etienne; Hijri, Mohamed; St-Arnaud, Marc.

In: Applied and environmental microbiology, Vol. 82, No. 18, 01.01.2016, p. 5530-5541.

Research output: Contribution to journalArticle

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