A Drosophila simulans mutant strain sensitive to benzo[a]pyrene and 2-acetylaminofluorene

S. Yu Fuchs, R. D. Safaev, E. M. Khovanova, H. G. Ugnivenko, V. S. Speigelman, T. A. Lytcheva, I. A. Khitrovo, G. A. Belitsky

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We have identified a Drosophila simulans mutant, 364 yv, that is sensitive to the toxic effects of the procarcinogens B(a)P and 2-AAF. Heterozygotes obtained by crossing it to the wild resistant Turku strain (♀ 364 yv × ♂ Turku) were more sensitive than heterozygotes obtained from the reciprocal cross (♀ Turku × ♂ 364yv) to both the toxic and the mutagenic effects of B(a)P in Drosophila tests that measured lethality and the induction of somatic mosaicism, respectively. The non-carcinogens pyrene, B(e)P and 4-AAF were only weakly toxic and non-mutagenic. In the Ames test B(a)P activation with S15 fractions prepared from the homogenates of Drosophila larvae and imagoes of the 364yv strain, as well as of the more resistant D. melanogaster y ++/+w sn3 heterozygotes, did not significantly increase the number of S. typhimurium TA100 revertants even following pretreatment with inducers of microsomal monooxygenases (B(a)P, PCB, PB). As for 2-AAF, a certain increase was observed following only PB, but not B(a)P pretreatment. Possible mechanisms of B(a)P and 2-AAF sensitivity of the 364yv strain, and perspectives on using it for monitoring genotoxic environmental pollutants, are discussed.

Original languageEnglish (US)
Pages (from-to)155-163
Number of pages9
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume268
Issue number1
DOIs
StatePublished - Jul 1992

Fingerprint

2-Acetylaminofluorene
Poisons
Benzo(a)pyrene
Heterozygote
Drosophila
Aryl Hydrocarbon Hydroxylases
Environmental Pollutants
Mosaicism
Polychlorinated Biphenyls
Larva
Drosophila simulans

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

Fuchs, S. Yu ; Safaev, R. D. ; Khovanova, E. M. ; Ugnivenko, H. G. ; Speigelman, V. S. ; Lytcheva, T. A. ; Khitrovo, I. A. ; Belitsky, G. A. / A Drosophila simulans mutant strain sensitive to benzo[a]pyrene and 2-acetylaminofluorene. In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 1992 ; Vol. 268, No. 1. pp. 155-163.
@article{d3f244d12d3f4441baaa9836266011e5,
title = "A Drosophila simulans mutant strain sensitive to benzo[a]pyrene and 2-acetylaminofluorene",
abstract = "We have identified a Drosophila simulans mutant, 364 yv, that is sensitive to the toxic effects of the procarcinogens B(a)P and 2-AAF. Heterozygotes obtained by crossing it to the wild resistant Turku strain (♀ 364 yv × ♂ Turku) were more sensitive than heterozygotes obtained from the reciprocal cross (♀ Turku × ♂ 364yv) to both the toxic and the mutagenic effects of B(a)P in Drosophila tests that measured lethality and the induction of somatic mosaicism, respectively. The non-carcinogens pyrene, B(e)P and 4-AAF were only weakly toxic and non-mutagenic. In the Ames test B(a)P activation with S15 fractions prepared from the homogenates of Drosophila larvae and imagoes of the 364yv strain, as well as of the more resistant D. melanogaster y ++/+w sn3 heterozygotes, did not significantly increase the number of S. typhimurium TA100 revertants even following pretreatment with inducers of microsomal monooxygenases (B(a)P, PCB, PB). As for 2-AAF, a certain increase was observed following only PB, but not B(a)P pretreatment. Possible mechanisms of B(a)P and 2-AAF sensitivity of the 364yv strain, and perspectives on using it for monitoring genotoxic environmental pollutants, are discussed.",
author = "Fuchs, {S. Yu} and Safaev, {R. D.} and Khovanova, {E. M.} and Ugnivenko, {H. G.} and Speigelman, {V. S.} and Lytcheva, {T. A.} and Khitrovo, {I. A.} and Belitsky, {G. A.}",
year = "1992",
month = "7",
doi = "10.1016/0027-5107(92)90092-G",
language = "English (US)",
volume = "268",
pages = "155--163",
journal = "Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis",
issn = "1386-1964",
publisher = "Elsevier BV",
number = "1",

}

A Drosophila simulans mutant strain sensitive to benzo[a]pyrene and 2-acetylaminofluorene. / Fuchs, S. Yu; Safaev, R. D.; Khovanova, E. M.; Ugnivenko, H. G.; Speigelman, V. S.; Lytcheva, T. A.; Khitrovo, I. A.; Belitsky, G. A.

In: Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, Vol. 268, No. 1, 07.1992, p. 155-163.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A Drosophila simulans mutant strain sensitive to benzo[a]pyrene and 2-acetylaminofluorene

AU - Fuchs, S. Yu

AU - Safaev, R. D.

AU - Khovanova, E. M.

AU - Ugnivenko, H. G.

AU - Speigelman, V. S.

AU - Lytcheva, T. A.

AU - Khitrovo, I. A.

AU - Belitsky, G. A.

PY - 1992/7

Y1 - 1992/7

N2 - We have identified a Drosophila simulans mutant, 364 yv, that is sensitive to the toxic effects of the procarcinogens B(a)P and 2-AAF. Heterozygotes obtained by crossing it to the wild resistant Turku strain (♀ 364 yv × ♂ Turku) were more sensitive than heterozygotes obtained from the reciprocal cross (♀ Turku × ♂ 364yv) to both the toxic and the mutagenic effects of B(a)P in Drosophila tests that measured lethality and the induction of somatic mosaicism, respectively. The non-carcinogens pyrene, B(e)P and 4-AAF were only weakly toxic and non-mutagenic. In the Ames test B(a)P activation with S15 fractions prepared from the homogenates of Drosophila larvae and imagoes of the 364yv strain, as well as of the more resistant D. melanogaster y ++/+w sn3 heterozygotes, did not significantly increase the number of S. typhimurium TA100 revertants even following pretreatment with inducers of microsomal monooxygenases (B(a)P, PCB, PB). As for 2-AAF, a certain increase was observed following only PB, but not B(a)P pretreatment. Possible mechanisms of B(a)P and 2-AAF sensitivity of the 364yv strain, and perspectives on using it for monitoring genotoxic environmental pollutants, are discussed.

AB - We have identified a Drosophila simulans mutant, 364 yv, that is sensitive to the toxic effects of the procarcinogens B(a)P and 2-AAF. Heterozygotes obtained by crossing it to the wild resistant Turku strain (♀ 364 yv × ♂ Turku) were more sensitive than heterozygotes obtained from the reciprocal cross (♀ Turku × ♂ 364yv) to both the toxic and the mutagenic effects of B(a)P in Drosophila tests that measured lethality and the induction of somatic mosaicism, respectively. The non-carcinogens pyrene, B(e)P and 4-AAF were only weakly toxic and non-mutagenic. In the Ames test B(a)P activation with S15 fractions prepared from the homogenates of Drosophila larvae and imagoes of the 364yv strain, as well as of the more resistant D. melanogaster y ++/+w sn3 heterozygotes, did not significantly increase the number of S. typhimurium TA100 revertants even following pretreatment with inducers of microsomal monooxygenases (B(a)P, PCB, PB). As for 2-AAF, a certain increase was observed following only PB, but not B(a)P pretreatment. Possible mechanisms of B(a)P and 2-AAF sensitivity of the 364yv strain, and perspectives on using it for monitoring genotoxic environmental pollutants, are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0026765362&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026765362&partnerID=8YFLogxK

U2 - 10.1016/0027-5107(92)90092-G

DO - 10.1016/0027-5107(92)90092-G

M3 - Article

C2 - 1378181

AN - SCOPUS:0026765362

VL - 268

SP - 155

EP - 163

JO - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

JF - Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

SN - 1386-1964

IS - 1

ER -