A fast degradable citrate-based bone scaffold promotes spinal fusion

Jiajun Tang, Jinshan Guo, Zhen Li, Cheng Yang, Denghui Xie, Jian Chen, Shengfa Li, Shaolin Li, Gloria B. Kim, Xiaochun Bai, Zhongmin Zhang, Jian Yang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

It is well known that high rates of fusion failure and pseudoarthrosis development (5-35%) are concomitant in spinal fusion surgery, which was ascribed to the shortage of suitable materials for bone regeneration. Citrate was recently recognized to play an indispensable role in enhancing osteoconductivity and osteoinductivity, and promoting bone formation. To address the material challenges in spinal fusion surgery, we have synthesized mechanically robust and fast degrading citrate-based polymers by incorporating N-methyldiethanolamine (MDEA) into clickable poly(1,8-octanediol citrates) (POC-click), referred to as POC-M-click. The obtained POC-M-click were fabricated into POC-M-click-HA matchstick scaffolds by forming composites with hydroxyapatite (HA) for interbody spinal fusion in a rabbit model. Spinal fusion was analyzed by radiography, manual palpation, biomechanical testing, and histological evaluation. At 4 and 8 weeks post surgery, POC-M-click-HA scaffolds showed optimal degradation rates that facilitated faster new bone formation and higher spinal fusion rates (11.2 ± 3.7, 80 ± 4.5 at week 4 and 8, respectively) than the poly(l-lactic acid)-HA (PLLA-HA) control group (9.3 ± 2.4 and 71.1 ± 4.4) (p < 0.05). The POC-M-click-HA scaffold-fused vertebrates possessed a maximum load and stiffness of 880.8 ± 14.5 N and 843.2 ± 22.4 N mm-1, respectively, which were also much higher than those of the PLLA-HA group (maximum: 712.0 ± 37.5 N, stiffness: 622.5 ± 28.4 N mm-1, p < 0.05). Overall, the results suggest that POC-M-click-HA scaffolds could potentially serve as promising bone grafts for spinal fusion applications.

Original languageEnglish (US)
Pages (from-to)5569-5576
Number of pages8
JournalJournal of Materials Chemistry B
Volume3
Issue number27
DOIs
StatePublished - Jul 21 2015

Fingerprint

Scaffolds
Citric Acid
Durapatite
Hydroxyapatite
Bone
Fusion reactions
Surgery
N-methyldiethanolamine
Lactic acid
Lactic Acid
Stiffness
Radiography
Grafts
Loads (forces)
Polymers
Degradation
Composite materials
Testing

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Tang, Jiajun ; Guo, Jinshan ; Li, Zhen ; Yang, Cheng ; Xie, Denghui ; Chen, Jian ; Li, Shengfa ; Li, Shaolin ; Kim, Gloria B. ; Bai, Xiaochun ; Zhang, Zhongmin ; Yang, Jian. / A fast degradable citrate-based bone scaffold promotes spinal fusion. In: Journal of Materials Chemistry B. 2015 ; Vol. 3, No. 27. pp. 5569-5576.
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title = "A fast degradable citrate-based bone scaffold promotes spinal fusion",
abstract = "It is well known that high rates of fusion failure and pseudoarthrosis development (5-35{\%}) are concomitant in spinal fusion surgery, which was ascribed to the shortage of suitable materials for bone regeneration. Citrate was recently recognized to play an indispensable role in enhancing osteoconductivity and osteoinductivity, and promoting bone formation. To address the material challenges in spinal fusion surgery, we have synthesized mechanically robust and fast degrading citrate-based polymers by incorporating N-methyldiethanolamine (MDEA) into clickable poly(1,8-octanediol citrates) (POC-click), referred to as POC-M-click. The obtained POC-M-click were fabricated into POC-M-click-HA matchstick scaffolds by forming composites with hydroxyapatite (HA) for interbody spinal fusion in a rabbit model. Spinal fusion was analyzed by radiography, manual palpation, biomechanical testing, and histological evaluation. At 4 and 8 weeks post surgery, POC-M-click-HA scaffolds showed optimal degradation rates that facilitated faster new bone formation and higher spinal fusion rates (11.2 ± 3.7, 80 ± 4.5 at week 4 and 8, respectively) than the poly(l-lactic acid)-HA (PLLA-HA) control group (9.3 ± 2.4 and 71.1 ± 4.4) (p < 0.05). The POC-M-click-HA scaffold-fused vertebrates possessed a maximum load and stiffness of 880.8 ± 14.5 N and 843.2 ± 22.4 N mm-1, respectively, which were also much higher than those of the PLLA-HA group (maximum: 712.0 ± 37.5 N, stiffness: 622.5 ± 28.4 N mm-1, p < 0.05). Overall, the results suggest that POC-M-click-HA scaffolds could potentially serve as promising bone grafts for spinal fusion applications.",
author = "Jiajun Tang and Jinshan Guo and Zhen Li and Cheng Yang and Denghui Xie and Jian Chen and Shengfa Li and Shaolin Li and Kim, {Gloria B.} and Xiaochun Bai and Zhongmin Zhang and Jian Yang",
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Tang, J, Guo, J, Li, Z, Yang, C, Xie, D, Chen, J, Li, S, Li, S, Kim, GB, Bai, X, Zhang, Z & Yang, J 2015, 'A fast degradable citrate-based bone scaffold promotes spinal fusion', Journal of Materials Chemistry B, vol. 3, no. 27, pp. 5569-5576. https://doi.org/10.1039/c5tb00607d

A fast degradable citrate-based bone scaffold promotes spinal fusion. / Tang, Jiajun; Guo, Jinshan; Li, Zhen; Yang, Cheng; Xie, Denghui; Chen, Jian; Li, Shengfa; Li, Shaolin; Kim, Gloria B.; Bai, Xiaochun; Zhang, Zhongmin; Yang, Jian.

In: Journal of Materials Chemistry B, Vol. 3, No. 27, 21.07.2015, p. 5569-5576.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A fast degradable citrate-based bone scaffold promotes spinal fusion

AU - Tang, Jiajun

AU - Guo, Jinshan

AU - Li, Zhen

AU - Yang, Cheng

AU - Xie, Denghui

AU - Chen, Jian

AU - Li, Shengfa

AU - Li, Shaolin

AU - Kim, Gloria B.

AU - Bai, Xiaochun

AU - Zhang, Zhongmin

AU - Yang, Jian

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Y1 - 2015/7/21

N2 - It is well known that high rates of fusion failure and pseudoarthrosis development (5-35%) are concomitant in spinal fusion surgery, which was ascribed to the shortage of suitable materials for bone regeneration. Citrate was recently recognized to play an indispensable role in enhancing osteoconductivity and osteoinductivity, and promoting bone formation. To address the material challenges in spinal fusion surgery, we have synthesized mechanically robust and fast degrading citrate-based polymers by incorporating N-methyldiethanolamine (MDEA) into clickable poly(1,8-octanediol citrates) (POC-click), referred to as POC-M-click. The obtained POC-M-click were fabricated into POC-M-click-HA matchstick scaffolds by forming composites with hydroxyapatite (HA) for interbody spinal fusion in a rabbit model. Spinal fusion was analyzed by radiography, manual palpation, biomechanical testing, and histological evaluation. At 4 and 8 weeks post surgery, POC-M-click-HA scaffolds showed optimal degradation rates that facilitated faster new bone formation and higher spinal fusion rates (11.2 ± 3.7, 80 ± 4.5 at week 4 and 8, respectively) than the poly(l-lactic acid)-HA (PLLA-HA) control group (9.3 ± 2.4 and 71.1 ± 4.4) (p < 0.05). The POC-M-click-HA scaffold-fused vertebrates possessed a maximum load and stiffness of 880.8 ± 14.5 N and 843.2 ± 22.4 N mm-1, respectively, which were also much higher than those of the PLLA-HA group (maximum: 712.0 ± 37.5 N, stiffness: 622.5 ± 28.4 N mm-1, p < 0.05). Overall, the results suggest that POC-M-click-HA scaffolds could potentially serve as promising bone grafts for spinal fusion applications.

AB - It is well known that high rates of fusion failure and pseudoarthrosis development (5-35%) are concomitant in spinal fusion surgery, which was ascribed to the shortage of suitable materials for bone regeneration. Citrate was recently recognized to play an indispensable role in enhancing osteoconductivity and osteoinductivity, and promoting bone formation. To address the material challenges in spinal fusion surgery, we have synthesized mechanically robust and fast degrading citrate-based polymers by incorporating N-methyldiethanolamine (MDEA) into clickable poly(1,8-octanediol citrates) (POC-click), referred to as POC-M-click. The obtained POC-M-click were fabricated into POC-M-click-HA matchstick scaffolds by forming composites with hydroxyapatite (HA) for interbody spinal fusion in a rabbit model. Spinal fusion was analyzed by radiography, manual palpation, biomechanical testing, and histological evaluation. At 4 and 8 weeks post surgery, POC-M-click-HA scaffolds showed optimal degradation rates that facilitated faster new bone formation and higher spinal fusion rates (11.2 ± 3.7, 80 ± 4.5 at week 4 and 8, respectively) than the poly(l-lactic acid)-HA (PLLA-HA) control group (9.3 ± 2.4 and 71.1 ± 4.4) (p < 0.05). The POC-M-click-HA scaffold-fused vertebrates possessed a maximum load and stiffness of 880.8 ± 14.5 N and 843.2 ± 22.4 N mm-1, respectively, which were also much higher than those of the PLLA-HA group (maximum: 712.0 ± 37.5 N, stiffness: 622.5 ± 28.4 N mm-1, p < 0.05). Overall, the results suggest that POC-M-click-HA scaffolds could potentially serve as promising bone grafts for spinal fusion applications.

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