A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase

Yifeng Wei, Bin Li, Divya Prakash, James Gregory Ferry, Sean J. Elliott, Jo Anne Stubbe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism.

Original languageEnglish (US)
Pages (from-to)7019-7028
Number of pages10
JournalBiochemistry
Volume54
Issue number47
DOIs
StatePublished - Nov 4 2015

Fingerprint

Methanosarcina barkeri
Ribonucleotide Reductases
Ferredoxins
Disulfides
Oxidoreductases
Electrons
Ribonucleotides
Thioredoxins
formic acid
Anaerobiosis
Thioredoxin-Disulfide Reductase
Oxidation
Cloning
Archaea
Operon
NADP
Metabolism
Organism Cloning
Catalytic Domain
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

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abstract = "Two subtypes of class III anaerobic ribonucleotide reductases (RNRs) studied so far couple the reduction of ribonucleotides to the oxidation of formate, or the oxidation of NADPH via thioredoxin and thioredoxin reductase. Certain methanogenic archaea contain a phylogenetically distinct third subtype of class III RNR, with distinct active-site residues. Here we report the cloning and recombinant expression of the Methanosarcina barkeri class III RNR and show that the electrons required for ribonucleotide reduction can be delivered by a [4Fe-4S] protein ferredoxin disulfide reductase, and a conserved thioredoxin-like protein NrdH present in the RNR operon. The diversity of class III RNRs reflects the diversity of electron carriers used in anaerobic metabolism.",
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A Ferredoxin Disulfide Reductase Delivers Electrons to the Methanosarcina barkeri Class III Ribonucleotide Reductase. / Wei, Yifeng; Li, Bin; Prakash, Divya; Ferry, James Gregory; Elliott, Sean J.; Stubbe, Jo Anne.

In: Biochemistry, Vol. 54, No. 47, 04.11.2015, p. 7019-7028.

Research output: Contribution to journalArticle

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AU - Wei, Yifeng

AU - Li, Bin

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AU - Ferry, James Gregory

AU - Elliott, Sean J.

AU - Stubbe, Jo Anne

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