A fiber-optic biosensor based on monooxygenases and sol-gel entrapped fluoresceinamine for trichloroethene and tetrachloroethene

D. Ryoo, Y. Kim, K. Jeun, T. Wood, Y. Choi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Directed evolution was performed for toluene-o-monooxygenase (TOM) and toluene/xylene-o-monooxygenase (ToMO) to obtain differential and improved reaction rates with respect to each chlorinated ethenes. Peroxide shunt was applied to remove the requirement for NADH, thus eliminating the need for complex NADH regeneration schemes for live cells on the biosensor tip and long-term application of enzyme biosensor would be possible. A three-layer sandwich structured sensing tip was constructed. The monooxygenases were immobilized on the outer layer, consisting of hydrophilic modified polyvinylidenefluoride membrane. The membrane was in contact with an intermediate sol-gel that incorporated fluoresceinamine (FLA), layered on an inner glass disk. The sensor operated in a static mode at room temperature and the intensity variation caused by hydrogen ion served as an analytical signal. Calibration curves were obtained for TCE and PCE, with concentration ranges 0.2-100 mg/l. The detection limits were 10μg/l for TCE and PCE. The method reproducibility was tested. The method was successfully applied to the detection and determination of these chlorinated ethenes in water samples, without sample preparation steps.

Original languageEnglish (US)
Title of host publication2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings
EditorsM. Laudon, B. Romanowicz
Pages505-507
Number of pages3
StatePublished - Dec 1 2005
Event2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 - Anaheim, CA, United States
Duration: May 8 2005May 12 2005

Publication series

Name2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005 Technical Proceedings

Other

Other2005 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2005
CountryUnited States
CityAnaheim, CA
Period5/8/055/12/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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