A field-deployable mobile molecular diagnostic system for malaria at the point of need

Gihoon Choi, Daniel Song, Sony Shrestha, Jun Miao, Liwang Cui, Weihua Guan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In response to the urgent need of a field-deployable and highly sensitive malaria diagnosis, we developed a standalone, "sample-in-answer-out" molecular diagnostic system (AnyMDx) to enable quantitative molecular analysis of blood-borne malaria in low resource areas. The system consists of a durable battery-powered analyzer and a disposable microfluidic compact disc loaded with reagents ready for use. A low power thermal module and a novel fluorescence-sensing module are integrated into the analyzer for real-time monitoring of loop-mediated isothermal nucleic acid amplification (LAMP) of target parasite DNA. With 10 μL of raw blood sample, the AnyMDx system automates the nucleic acid sample preparation and subsequent LAMP and real-time detection. Under laboratory conditions with whole-blood samples spiked with cultured Plasmodium falciparum, we achieved a detection limit of ∼0.6 parasite per μL, much lower than those for the conventional microscopy and rapid diagnostic tests (∼50-100 parasites per μL). The turnaround time from sample to answer is less than 40 minutes. The AnyMDx is user-friendly requiring minimal technological training. The analyzer and the disposable reagent compact discs are cost-effective, making AnyMDx a potential tool for malaria molecular diagnosis under field settings for malaria elimination.

Original languageEnglish (US)
Pages (from-to)4341-4349
Number of pages9
JournalLab on a Chip
Volume16
Issue number22
DOIs
StatePublished - Jan 1 2016

Fingerprint

Molecular Pathology
Malaria
Blood
Nucleic acids
Nucleic Acids
Parasites
Turnaround time
Microfluidics
Amplification
Microscopic examination
DNA
Plasmodium falciparum
Fluorescence
Routine Diagnostic Tests
Limit of Detection
Microscopy
Monitoring
Hot Temperature
Costs and Cost Analysis
Costs

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Choi, Gihoon ; Song, Daniel ; Shrestha, Sony ; Miao, Jun ; Cui, Liwang ; Guan, Weihua. / A field-deployable mobile molecular diagnostic system for malaria at the point of need. In: Lab on a Chip. 2016 ; Vol. 16, No. 22. pp. 4341-4349.
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A field-deployable mobile molecular diagnostic system for malaria at the point of need. / Choi, Gihoon; Song, Daniel; Shrestha, Sony; Miao, Jun; Cui, Liwang; Guan, Weihua.

In: Lab on a Chip, Vol. 16, No. 22, 01.01.2016, p. 4341-4349.

Research output: Contribution to journalArticle

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