A first analysis of metallome biosignatures of hyperthermophilic archaea

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

To date, no experimental data has been reported for the metallome of hyperthermophilic microorganisms although their metal requirements for growth are known to be unique. Here, experiments were conducted to determine (i) cellular trace metal concentrations of the hyperthermophilic Archaea Methanococcus jannaschii and Pyrococcus furiosus, and (ii) a first estimate of the metallome for these hyperthermophilic species via ICP-MS. The metal contents of these cells were compared to parallel experiments using the mesophilic bacterium Escherichia coli grown under aerobic and anaerobic conditions. Fe and Zn were typically the most abundant metals in cells. Metal concentrations for E. coli grown aerobically decreased in the order Fe > Zn > Cu > Mo > Ni > W > Co. In contrast, M. jannaschii and P. furiosus show almost the reverse pattern with elevated Ni, Co, and W concentrations. Of the three organisms, a biosignature is potentially demonstrated for the methanogen M. jannaschii that may, in part, be related to the metallome requirements of methanogenesis. The bioavailability of trace metals more than likely has varied through time. If hyperthermophiles are very ancient, then the trace metal patterns observed here may begin to provide some insights regarding Earth's earliest cells and in turn, early Earth chemistry.

Original languageEnglish (US)
Article number789278
JournalArchaea
Volume2012
DOIs
StatePublished - 2012

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Physiology
  • Ecology, Evolution, Behavior and Systematics

Fingerprint Dive into the research topics of 'A first analysis of metallome biosignatures of hyperthermophilic archaea'. Together they form a unique fingerprint.

Cite this