A first analysis of metallome biosignatures of hyperthermophilic archaea

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To date, no experimental data has been reported for the metallome of hyperthermophilic microorganisms although their metal requirements for growth are known to be unique. Here, experiments were conducted to determine (i) cellular trace metal concentrations of the hyperthermophilic Archaea Methanococcus jannaschii and Pyrococcus furiosus, and (ii) a first estimate of the metallome for these hyperthermophilic species via ICP-MS. The metal contents of these cells were compared to parallel experiments using the mesophilic bacterium Escherichia coli grown under aerobic and anaerobic conditions. Fe and Zn were typically the most abundant metals in cells. Metal concentrations for E. coli grown aerobically decreased in the order Fe > Zn > Cu > Mo > Ni > W > Co. In contrast, M. jannaschii and P. furiosus show almost the reverse pattern with elevated Ni, Co, and W concentrations. Of the three organisms, a biosignature is potentially demonstrated for the methanogen M. jannaschii that may, in part, be related to the metallome requirements of methanogenesis. The bioavailability of trace metals more than likely has varied through time. If hyperthermophiles are very ancient, then the trace metal patterns observed here may begin to provide some insights regarding Earth's earliest cells and in turn, early Earth chemistry.

Original languageEnglish (US)
Article number789278
JournalArchaea
Volume2012
DOIs
StatePublished - Dec 27 2012

Fingerprint

Archaea
Pyrococcus furiosus
Metals
metals
trace metal
trace elements
Methanocaldococcus
early Earth
metal
Methanococcus
Escherichia coli
methanogens
methanogenesis
aerobic conditions
cells
methane production
oxic conditions
anaerobic conditions
anoxic conditions
bioavailability

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Physiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

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A first analysis of metallome biosignatures of hyperthermophilic archaea. / Cameron, Vyllinniskii; House, Christopher H.; Brantley, Susan L.

In: Archaea, Vol. 2012, 789278, 27.12.2012.

Research output: Contribution to journalArticle

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