A flexible and highly sensitive organic electrochemical transistor-based biosensor for continuous and wireless nitric oxide detection

Yuping Deng, Hui Qi, Yuan Ma, Shangbin Liu, Mingyou Zhao, Zhenhu Guo, Yongsheng Jie, Rui Zheng, Jinzhu Jing, Kuntao Chen, He Ding, Guoqing Lv, Kaiyuan Zhang, Rongfeng Li, Huanyu Cheng, Lingyun Zhao, Xing Sheng, Milin Zhang, Lan Yin

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

As nitric oxide (NO) plays significant roles in a variety of physiological processes, the capability for real-time and accurate detection of NO in live organisms is in great demand. Traditional assessments of NO rely on indirect colorimetric techniques or electrochemical sensors that often comprise rigid constituent materials and can hardly satisfy sensitivity and spatial resolution simultaneously. Here, we report a flexible and highly sensitive biosensor based on organic electrochemical transistors (OECTs) capable of continuous and wireless detection of NO in biological systems. By modifying the geometry of the active channel and the gate electrodes of OECTs, devices achieve optimum signal amplification of NO. The sensor exhibits a low response limit, a wide linear range, high sensitivity, and excellent selectivity, with a miniaturized active sensing region compared with a conventional electrochemical sensor. The device demonstrates continuous detection of the nanomolar range of NO in cultured cells for hours without significant signal drift. Real-time and wireless measurement of NO is accomplished for 8 d in the articular cavity of New Zealand White rabbits with anterior cruciate ligament (ACL) rupture injuries. The observed high level of NO is associated with the onset of osteoarthritis (OA) at the later stage. The proposed device platform could provide critical information for the early diagnosis of chronic diseases and timely medical intervention to optimize therapeutic efficacy.

Original languageEnglish (US)
Article numbere2208060119
JournalProceedings of the National Academy of Sciences of the United States of America
Volume119
Issue number34
DOIs
StatePublished - Aug 23 2022

All Science Journal Classification (ASJC) codes

  • General

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