A framework for collaborative analysis of ENCODE data: Making large-scale analyses biologist-friendly

Daniel Blankenberg, James Taylor, Ian Schenck, Jianbin He, Yi Zhang, Matthew Ghent, Narayanan Veeraraghavan, Istvan Albert, Webb Miller, Kateryna D. Makova, Ross C. Hardison, Anton Nekrutenko

Research output: Contribution to journalArticle

107 Scopus citations

Abstract

The standardization and sharing of data and tools are the biggest challenges of large collaborative projects such as the Encyclopedia of DNA Elements (ENCODE). Here we describe a compact Web application, Galaxy2 ENCODE, that effectively addresses these issues. It provides an intuitive interface for the deposition and access of data, and features a vast number of analysis tools including operations on genomic intervals, utilities for manipulation of multiple sequence alignments, and molecular evolution algorithms. By providing a direct link between data and analysis tools, Galaxy2ENCODE allows addressing biological questions that are beyond the reach of existing software. We use Galaxy2ENCODE to show that the ENCODE regions contain >2000 unannotated transcripts under strong purifying selection that are likely functional. We also show that the ENCODE regions are representative of the entire genome by estimating the rate of nucleotide substitution and comparing it to published data. Although each of these analyses is complex, none takes more than 15 min from beginning to end. Finally, we demonstrate how new tools can be added to Galaxy2ENCODE with almost no effort. Every section of the manuscript is supplemented with QuickTime screencasts. Galaxy2ENCODE and the screencasts can be accessed at http://g2.bx.psu.edu.

Original languageEnglish (US)
Pages (from-to)960-964
Number of pages5
JournalGenome research
Volume17
Issue number6
DOIs
StatePublished - Jun 1 2007

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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