A framework physical map for peach, a model Rosaceae species

T. N. Zhebentyayeva, G. Swire-Clark, L. L. Georgi, L. Garay, S. Jung, S. Forrest, A. V. Blenda, B. Blackmon, J. Mook, R. Horn, W. Howad, P. Arús, D. Main, J. P. Tomkins, B. Sosinski, W. V. Baird, G. L. Reighard, A. G. Abbott

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59 Scopus citations

Abstract

A genome-wide framework physical map of peach was constructed using high-information content fingerprinting (HICF) and FPC software. The resulting HICF assembly contained 2,138 contigs composed of 15,655 clones (4.3x peach genome equivalents) from two complementary bacterial artificial chromosome libraries. The total physical length of all contigs is estimated at 303 Mb or 104.5% of the peach genome. The framework physical map is anchored on the Prunus genetic reference map and integrated with the peach transcriptome map. The physical length of anchored contigs is estimated at 45.0 Mb or 15.5% of the genome. Altogether, 2,636 markers, i.e., genetic markers, peach unigene expressed sequence tags, and gene-specific and overgo probes, were incorporated into the physical framework and supported the accuracy of contig assembly.

Original languageEnglish (US)
Pages (from-to)745-756
Number of pages12
JournalTree Genetics and Genomes
Volume4
Issue number4
DOIs
Publication statusPublished - Oct 1 2008

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All Science Journal Classification (ASJC) codes

  • Forestry
  • Molecular Biology
  • Genetics
  • Horticulture

Cite this

Zhebentyayeva, T. N., Swire-Clark, G., Georgi, L. L., Garay, L., Jung, S., Forrest, S., ... Abbott, A. G. (2008). A framework physical map for peach, a model Rosaceae species. Tree Genetics and Genomes, 4(4), 745-756. https://doi.org/10.1007/s11295-008-0147-z