A functional imaging study of germinating oilseed rape seed

Eberhard Munz, Hardy Rolletschek, Steffen Oeltze-Jafra, Johannes Fuchs, André Guendel, Thomas Neuberger, Stefan Ortleb, Peter M. Jakob, Ljudmilla Borisjuk

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Germination, the process whereby a dry, quiescent seed springs to life, has been a focus of plant biologist for many years, yet the early events following water uptake, during which metabolism of the embryo is restarted, remain enigmatic. Here, the nature of the cues required for this restarting in oilseed rape (Brassica napus) seed has been investigated. A holistic in vivo approach was designed to display the link between the entry and allocation of water, metabolic events and structural changes occurring during germination. For this, we combined functional magnetic resonance imaging with Fourier transform infrared microscopy, fluorescence-based respiration mapping, computer-aided seed modeling and biochemical tools. We uncovered an endospermal lipid gap, which channels water to the radicle tip, from whence it is distributed via embryonic vasculature toward cotyledon tissues. The resumption of respiration is initiated first in the endosperm, only later spreading to the embryo. Sugar metabolism and lipid utilization are linked to the spatiotemporal sequence of tissue rehydration. Together, this imaging study provides insights into the spatial aspects of key events in oilseed rape seeds leading to germination. It demonstrates how seed architecture predetermines the pattern of water intake, which sets the stage for the orchestrated restart of life.

Original languageEnglish (US)
Pages (from-to)1181-1190
Number of pages10
JournalNew Phytologist
Volume216
Issue number4
DOIs
StatePublished - Dec 1 2017

Fingerprint

rapeseed
Brassica napus
Seeds
image analysis
Germination
germination
seeds
embryo (plant)
root radicle
Respiration
water allocation
Embryonic Structures
metabolism
rehydration
lipids
fluorescence microscopy
Endosperm
Aquaporins
magnetic resonance imaging
water uptake

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

Munz, E., Rolletschek, H., Oeltze-Jafra, S., Fuchs, J., Guendel, A., Neuberger, T., ... Borisjuk, L. (2017). A functional imaging study of germinating oilseed rape seed. New Phytologist, 216(4), 1181-1190. https://doi.org/10.1111/nph.14736
Munz, Eberhard ; Rolletschek, Hardy ; Oeltze-Jafra, Steffen ; Fuchs, Johannes ; Guendel, André ; Neuberger, Thomas ; Ortleb, Stefan ; Jakob, Peter M. ; Borisjuk, Ljudmilla. / A functional imaging study of germinating oilseed rape seed. In: New Phytologist. 2017 ; Vol. 216, No. 4. pp. 1181-1190.
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Munz, E, Rolletschek, H, Oeltze-Jafra, S, Fuchs, J, Guendel, A, Neuberger, T, Ortleb, S, Jakob, PM & Borisjuk, L 2017, 'A functional imaging study of germinating oilseed rape seed', New Phytologist, vol. 216, no. 4, pp. 1181-1190. https://doi.org/10.1111/nph.14736

A functional imaging study of germinating oilseed rape seed. / Munz, Eberhard; Rolletschek, Hardy; Oeltze-Jafra, Steffen; Fuchs, Johannes; Guendel, André; Neuberger, Thomas; Ortleb, Stefan; Jakob, Peter M.; Borisjuk, Ljudmilla.

In: New Phytologist, Vol. 216, No. 4, 01.12.2017, p. 1181-1190.

Research output: Contribution to journalArticle

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Munz E, Rolletschek H, Oeltze-Jafra S, Fuchs J, Guendel A, Neuberger T et al. A functional imaging study of germinating oilseed rape seed. New Phytologist. 2017 Dec 1;216(4):1181-1190. https://doi.org/10.1111/nph.14736