A gapmer aptamer nanobiosensor for real-time monitoring of transcription and translation in single cells

Shue Wang, Yuan Xiao, Donna D. Zhang, Pak Kin Wong

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transcription and translation are under tight spatiotemporal regulation among cells to coordinate multicellular organization. Methods that allow massively parallel detection of gene expression dynamics at the single cell level are required for elucidating the complex regulatory mechanisms. Here we present a multiplex nanobiosensor for real-time monitoring of protein and mRNA expression dynamics in live cells based on gapmer aptamers and complementary locked nucleic acid probes. Using the multiplex nanobiosensor, we quantified spatiotemporal dynamics of vascular endothelial growth factor A mRNA and protein expressions in single human endothelial cells during microvascular self-organization. Our results revealed distinct gene regulatory processes in the heterogeneous cell subpopulations.

Original languageEnglish (US)
Pages (from-to)56-64
Number of pages9
JournalBiomaterials
Volume156
DOIs
StatePublished - Feb 1 2018

Fingerprint

Transcription
Monitoring
Nucleic Acid Probes
Proteins
Messenger RNA
Nucleic acids
Endothelial cells
Gene expression
Vascular Endothelial Growth Factor A
Regulator Genes
Genes
Endothelial Cells
Gene Expression

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

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A gapmer aptamer nanobiosensor for real-time monitoring of transcription and translation in single cells. / Wang, Shue; Xiao, Yuan; Zhang, Donna D.; Wong, Pak Kin.

In: Biomaterials, Vol. 156, 01.02.2018, p. 56-64.

Research output: Contribution to journalArticle

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