A gas chromatography-isotope dilution high-resolution mass spectrometry method for quantification of isomeric benzo[a]pyrene diol epoxide hemoglobin adducts in humans

Angela D. Ragin, Kenroy E. Crawford, Alisha A. Etheredge, James Grainger, Donald G. Patterson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

We developed a gas chromatography-isotope dilution high-resolution mass spectrometry (GC-ID-HRMS) method for quantifying isomers of benzo[a]pyrene (BaP) tetrol metabolites resulting from hydrolysis of benzo[a]pyrene-diol-epoxide hemoglobin (BaPDE-Hb) adducts. Acid hydrolysis of BPDE-Hb adducts extracted from human blood samples yielded isomers of benzo[a]pyrene-tetrahydrotetrols, (±)-BaP-r-7,t-8,t-9,c-10-tetrol (BPTI-1), (±)-BaP-r-7,t-8,t-9,t- 10-tetrol (BPTI-2), (±)-BaP-r-7,t-8,c-9,t-10-tetrol (BPTII-1), and (±)-BaP-r-7,t-8,c-9,c-10-tetrol (BPTII-2). The isomeric BaP tetrols were isolated from the matrix by liquid-liquid extraction, and then further purified by solid-phase extraction. Following silylation with N-methyl-N-(trimethylsilyl) -trifluoroacetamide, the analytes were measured by GC-HRMS, using electron ionization. We have found detectable concentrations in the low fmol range for BPTII-1 and BPTI-1 in all donors tested. The mean BaP adduct levels for smoking donors (n = 9) were 0.022 fmol/mg hemoglobin for BPTII-1 and 0.070 fmol/mg hemoglobin for BPTI-1. The mean BaP adduct levels with hemoglobin for non smoking donors (n = 6) was 0.021 fmol/mg hemoglobin for BPTII-1 and 0.105 fmol/mg hemoglobin for BPTI-1.

Original languageEnglish (US)
Pages (from-to)728-736
Number of pages9
JournalJournal of Analytical Toxicology
Volume32
Issue number9
DOIs
StatePublished - Jan 1 2008

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Environmental Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis
  • Chemical Health and Safety

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