A GATA-1-regulated microRNA locus essential for erythropoiesis

Louis C. Dore, Julio D. Amigo, Camila O. Dos Santos, Zhe Zhang, Xiaowu Gai, John W. Tobias, Duonan Yu, Alyssa M. Klein, Christine Dorman, Weisheng Wu, Ross Cameron Hardison, Barry H. Paw, Mitchell J. Weiss

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) control tissue development, but their mechanism of regulation is not well understood. We used a gene complementation strategy combined with microarray screening to identify miRNAs involved in the formation of erythroid (red blood) cells. Two conserved miRNAs, miR 144 and miR 451, emerged as direct targets of the critical hematopoietic transcription factor GATA-1. In vivo, GATA-1 binds a distal upstream regulatory element to activate RNA polymerase II-mediated transcription of a single common precursor RNA (pri-miRNA) encoding both mature miRNAs. Zebrafish embryos depleted of miR 451 by using antisense morpholinos form erythroid precursors, but their development into mature circulating red blood cells is strongly and specifically impaired. These results reveal a miRNA locus that is required for erythropoiesis and uncover a new regulatory axis through which GATA-1 controls this process.

Original languageEnglish (US)
Pages (from-to)3333-3338
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number9
DOIs
StatePublished - Mar 4 2008

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Erythropoiesis
MicroRNAs
Erythrocytes
GATA Transcription Factors
Morpholinos
RNA Polymerase II
RNA Precursors
Zebrafish
Embryonic Structures
Genes

All Science Journal Classification (ASJC) codes

  • General

Cite this

Dore, L. C., Amigo, J. D., Dos Santos, C. O., Zhang, Z., Gai, X., Tobias, J. W., ... Weiss, M. J. (2008). A GATA-1-regulated microRNA locus essential for erythropoiesis. Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3333-3338. https://doi.org/10.1073/pnas.0712312105
Dore, Louis C. ; Amigo, Julio D. ; Dos Santos, Camila O. ; Zhang, Zhe ; Gai, Xiaowu ; Tobias, John W. ; Yu, Duonan ; Klein, Alyssa M. ; Dorman, Christine ; Wu, Weisheng ; Hardison, Ross Cameron ; Paw, Barry H. ; Weiss, Mitchell J. / A GATA-1-regulated microRNA locus essential for erythropoiesis. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 9. pp. 3333-3338.
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Dore, LC, Amigo, JD, Dos Santos, CO, Zhang, Z, Gai, X, Tobias, JW, Yu, D, Klein, AM, Dorman, C, Wu, W, Hardison, RC, Paw, BH & Weiss, MJ 2008, 'A GATA-1-regulated microRNA locus essential for erythropoiesis', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 9, pp. 3333-3338. https://doi.org/10.1073/pnas.0712312105

A GATA-1-regulated microRNA locus essential for erythropoiesis. / Dore, Louis C.; Amigo, Julio D.; Dos Santos, Camila O.; Zhang, Zhe; Gai, Xiaowu; Tobias, John W.; Yu, Duonan; Klein, Alyssa M.; Dorman, Christine; Wu, Weisheng; Hardison, Ross Cameron; Paw, Barry H.; Weiss, Mitchell J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 9, 04.03.2008, p. 3333-3338.

Research output: Contribution to journalArticle

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AU - Dore, Louis C.

AU - Amigo, Julio D.

AU - Dos Santos, Camila O.

AU - Zhang, Zhe

AU - Gai, Xiaowu

AU - Tobias, John W.

AU - Yu, Duonan

AU - Klein, Alyssa M.

AU - Dorman, Christine

AU - Wu, Weisheng

AU - Hardison, Ross Cameron

AU - Paw, Barry H.

AU - Weiss, Mitchell J.

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