A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae

Ralph Keil, A. D. McWilliams

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The preservation of sequence homogeneity and copy number of tandemly repeated genes may require specific mechanisms or regulation of recombination. We have identified mutations that specifically affect recombination among natural repetitions in the yeast Saccharomyces cerevisiae. The rrm3 mutation stimulates mitotic recombination in the naturally occurring tandem repeats of the rDNA and copper chelatin (CUP1) genes. This mutation does not affect recombination of several other types of repeated genes tested including Ty elements, mating type information and duplications created by transformation. In addition to stimulating exchange among the multiple CUP1 repeats at their natural chromosomal location, rrm3 also increases recombination of a duplication of CUP1 units present at his4. This suggests that the RRM3 gene may encode a sequence-specific factor that contributes to a global suppression of mitotic exchange in sequences that can be maintained as tandem arrays.

Original languageEnglish (US)
Pages (from-to)711-718
Number of pages8
JournalGenetics
Volume135
Issue number3
StatePublished - Jan 1 1993

Fingerprint

Genetic Recombination
Saccharomyces cerevisiae
Genes
Mutation
Tandem Repeat Sequences
Ribosomal DNA
Copper
Yeasts

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

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A gene with specific and global effects on recombination of sequences from tandemly repeated genes in Saccharomyces cerevisiae. / Keil, Ralph; McWilliams, A. D.

In: Genetics, Vol. 135, No. 3, 01.01.1993, p. 711-718.

Research output: Contribution to journalArticle

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