A genome-wide housekeeping role for TFIID and a highly regulated stress-related role for SAGA in Saccharomyces cerevisiae

Kathryn L. Huisinga, Benjamin Franklin Pugh

Research output: Contribution to journalArticle

354 Citations (Scopus)

Abstract

TFIID and SAGA share a common set of TAFs, regulate chromatin, and deliver TBP to promoters. Here we examine their relationship within the context of the Saccharomyces cerevisiae genome-wide regulatory network. We find that while TFIID and SAGA make overlapping contributions to the expression of all genes, TFIID function predominates at ∼90% and SAGA at ∼10% of the measurable genome. Strikingly, SAGA-dominated genes are largely stress induced and TAF independent, and are downregulated by the coordinate action of a variety of chromatin, TBP, and RNA polymerase II regulators. In contrast, the TFIID-dominated class is less regulated, but is highly dependent upon TAFs, including those shared between TFIID and SAGA. These two distinct modes of transcription regulation might reflect the need to balance inducible stress responses with the steady output of housekeeping genes.

Original languageEnglish (US)
Pages (from-to)573-585
Number of pages13
JournalMolecular Cell
Volume13
Issue number4
DOIs
StatePublished - Feb 27 2004

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Transcription Factor TFIID
Housekeeping
Saccharomyces cerevisiae
Genome
Chromatin
RNA Polymerase II
Essential Genes
Down-Regulation
Gene Expression
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

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A genome-wide housekeeping role for TFIID and a highly regulated stress-related role for SAGA in Saccharomyces cerevisiae. / Huisinga, Kathryn L.; Pugh, Benjamin Franklin.

In: Molecular Cell, Vol. 13, No. 4, 27.02.2004, p. 573-585.

Research output: Contribution to journalArticle

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