A guanine nucleotide regulatory protein controls polyphosphoinositide metabolism, Ca2+ mobilization, and cellular responses to chemoattractants in human monocytes

M. W. Verghese, C. D. Smith, L. A. Charles, L. Jakoi, R. Synderman

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Previous studies demonstrated that oligopeptide chemoattractant receptors on PMN and macrophages exist in high and low affinity states which are interconvertible by guanosine di- and triphosphates. These observations suggest that guanine nucleotide regulatory (N) proteins play a role in phagocyte activation by chemotactic factors. The data presented here indicate that chemotactic factor receptors on monocytes utilize an N protein to activate phospholipase C and subsequent biologic responses by the cells. This conclusion is based on the findings that inactivation of an N protein of 41,000 m.w. by Bordetella pertussis toxin (PT) treatment abolishes monocyte responsiveness to chemoattractants but not to lectins, PMA, or the Ca2+ ionophore A23187. Treatment with PT inhibited IP3 production, Ca2+ mobilization, and cellular activation as assessed by chemotaxis and changes in forward light scattering in response to the chemoattractants by at least 80%. Therefore, a PT-sensitive N protein plays an important role in the activation of monocytes by chemoattractants.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalJournal of Immunology
Volume137
Issue number1
StatePublished - Sep 10 1986

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'A guanine nucleotide regulatory protein controls polyphosphoinositide metabolism, Ca<sup>2+</sup> mobilization, and cellular responses to chemoattractants in human monocytes'. Together they form a unique fingerprint.

  • Cite this