A high-density genetic map of tetraploid salix matsudana using specific length amplified fragment sequencing (SLAF-seq)

Jian Zhang, Huwei Yuan, Min Li, Yujuan Li, Ying Wang, Xiangjian Ma, Yuan Zhang, Feng Tan, Rongling Wu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

As a salt-tolerant arbor tree species, Salix matsudana plays an important role in afforestation and greening in the coastal areas of China. To select superior Salix varieties that adapt to wide saline areas, it is of paramount importance to understand and identify the mechanisms of salt-tolerance at the level of the whole genome. Here, we describe a high-density genetic linkage map of S. matsudana that represents a good coverage of the Salix genome. An intraspecific F1 hybrid population was established by crossing the salt-sensitive "Yanjiang" variety as the female parent with the salt-tolerant "9901" variety as the male parent. This population, along with its parents, was genotyped by specific length amplified fragment sequencing (SLAF-seq), leading to 277,333 high-quality SLAF markers. By marker analysis, we found that both the parents and offspring were tetraploid. The mean sequencing depth was 53.20-fold for "Yanjiang", 47.41-fold for "9901", and 11.02-fold for the offspring. Of the SLAF markers detected, 42,321 are polymorphic with sufficient quality for map construction. The final genetic map was constructed using 6,737 SLAF markers, covering 38 linkage groups (LGs). The genetic map spanned 5,497.45 cM in length, with an average distance of 0.82 cM. As a first high-density genetic map of S. matsudana constructed from salt tolerance-varying varieties, this study will provide a foundation for mapping quantitative trait loci that modulate salt tolerance and resistance in Salix and provide important references for molecular breeding of this important forest tree.

Original languageEnglish (US)
Article numbere0157777
JournalPLoS One
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Salix matsudana
Salix
Tetraploidy
tetraploidy
Salts
salt tolerance
Salt-Tolerance
salts
genome
afforestation
Genes
forest trees
linkage groups
DNA Shuffling
Genome
chromosome mapping
quantitative trait loci
Genetic Linkage
Quantitative Trait Loci
Population

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Zhang, Jian ; Yuan, Huwei ; Li, Min ; Li, Yujuan ; Wang, Ying ; Ma, Xiangjian ; Zhang, Yuan ; Tan, Feng ; Wu, Rongling. / A high-density genetic map of tetraploid salix matsudana using specific length amplified fragment sequencing (SLAF-seq). In: PLoS One. 2016 ; Vol. 11, No. 6.
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A high-density genetic map of tetraploid salix matsudana using specific length amplified fragment sequencing (SLAF-seq). / Zhang, Jian; Yuan, Huwei; Li, Min; Li, Yujuan; Wang, Ying; Ma, Xiangjian; Zhang, Yuan; Tan, Feng; Wu, Rongling.

In: PLoS One, Vol. 11, No. 6, e0157777, 01.06.2016.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Zhang, Jian

AU - Yuan, Huwei

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AU - Wang, Ying

AU - Ma, Xiangjian

AU - Zhang, Yuan

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AU - Wu, Rongling

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