A hybrid electrokinetic processor for isolating exfoliated cancer cells and circulating tumor cells in physiological samples

Jian Gao, Reza Riahi, Mandy L.Y. Sin, Pak Kin Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a hybrid electrokinetic processor that combines electrophoresis (EP), dielectrophoresis (DEP) and AC electrothermal flow (ACEF) for isolating exfoliated cancer cells (ECCs) and circulating tumor cells (CTCs) in physiological samples. While most electrokinetic techniques only function in low-conductivity buffers, hybrid electrokinetics enables effective operation in high-conductivity physiological fluids (∼ 1 S/m) such as blood, buffy coat and urine. Using a 3-electrode configuration with optimized AC voltage, frequency and DC offset, we demonstrate that breast cancer cells can be isolated in buffy coats and biological fluids in 10 sec.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1472-1474
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Tumors
Cells
Electrophoresis
Fluids
Blood
Electrodes
Electric potential

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Gao, J., Riahi, R., Sin, M. L. Y., & Wong, P. K. (2011). A hybrid electrokinetic processor for isolating exfoliated cancer cells and circulating tumor cells in physiological samples. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1472-1474). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).
Gao, Jian ; Riahi, Reza ; Sin, Mandy L.Y. ; Wong, Pak Kin. / A hybrid electrokinetic processor for isolating exfoliated cancer cells and circulating tumor cells in physiological samples. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1472-1474 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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Gao, J, Riahi, R, Sin, MLY & Wong, PK 2011, A hybrid electrokinetic processor for isolating exfoliated cancer cells and circulating tumor cells in physiological samples. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 3, pp. 1472-1474, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.

A hybrid electrokinetic processor for isolating exfoliated cancer cells and circulating tumor cells in physiological samples. / Gao, Jian; Riahi, Reza; Sin, Mandy L.Y.; Wong, Pak Kin.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1472-1474 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Gao J, Riahi R, Sin MLY, Wong PK. A hybrid electrokinetic processor for isolating exfoliated cancer cells and circulating tumor cells in physiological samples. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1472-1474. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).