A kinesin mutant with an atypical bipolar spindle undergoes normal mitosis

A. I. Marcus, W. Li, H. Ma, R. J. Cyr

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Motor proteins have been implicated in various aspects of mitosis, including spindle assembly and chromosome segregation. Here, we show that acentrosomal Arabidopsis cells that are mutant for the kinesin, ATK1, lack microtubule accumulation at the predicted spindle poles during prophase and have reduced spindle bipolarity during prometaphase. Nonetheless, all abnormalities are rectified by anaphase and chromosome segregation appears normal. We conclude that ATK1 is required for normal microtubule accumulation at the spindle poles during prophase and possibly functions in spindle assembly during prometaphase. Because aberrant spindle morphology in these mutants is resolved by anaphase, we postulate that mitotic plant cells contain an error-correcting mechanism. Moreover, ATK1 function seems to be dosage-dependent, because cells containing one wild-type allele take significantly longer to proceed to anaphase as compared with cells containing two wild-type alleles.

Original languageEnglish (US)
Pages (from-to)1717-1726
Number of pages10
JournalMolecular biology of the cell
Volume14
Issue number4
DOIs
StatePublished - Apr 1 2003

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Kinesin
Anaphase
Mitosis
Prometaphase
Spindle Poles
Prophase
Chromosome Segregation
Microtubules
Alleles
Plant Cells
Arabidopsis
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Marcus, A. I. ; Li, W. ; Ma, H. ; Cyr, R. J. / A kinesin mutant with an atypical bipolar spindle undergoes normal mitosis. In: Molecular biology of the cell. 2003 ; Vol. 14, No. 4. pp. 1717-1726.
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A kinesin mutant with an atypical bipolar spindle undergoes normal mitosis. / Marcus, A. I.; Li, W.; Ma, H.; Cyr, R. J.

In: Molecular biology of the cell, Vol. 14, No. 4, 01.04.2003, p. 1717-1726.

Research output: Contribution to journalArticle

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