Abstract
To determine the incidence of pS3 mutations in pre‐malignant lesions of the oral cavity from individuals without prior history of tobacco use, we have analyzed the conserved regions of the pS3 gene (exons 5‐9) in archival oral cavity lesion specimens obtained from patients with varied tobacco use histories, by polymerase chain reaction/single strand conformational polymorphism (PCR/SSCP) and DNA sequencing analysis. Twenty‐six lesions were analyzed from 14 patients, with multiple lesions obtained from 8 patients. Six of these patients used tobacco, (3 being cigarette smokers, 1 ex‐cigarette smoker, 1 moderate cigar smoker and 1 snuff chewer). The remaining 8 patients had no prior history of tobacco use. Thirteen of the pre‐malignant lesions exhibited severe dysplasia, 9 exhibited moderate dysplasia and 4 exhibited mild dysplasia. Four of the 26 lesions exhibited pS3 mutations, each being from a tobacco user. None of the 13 lesions from never‐tobacco users exhibited pS3 mutations. There was a significantly higher p53 mutation incidence in pre‐malignant lesions from tobacco users (including ex‐smokers) than in non‐tobacco users as well as in cigarette smokers plus snuff chewers than in non‐tobacco users. Two of the mutations were observed in lesions exhibiting severe dysplasia: I in a lesion exhibiting moderate dysplasia and I in a lesion exhibiting mild dysplasia. These data suggest that pS3 mutation may be a very early event in oral cavity tumor progression and demonstrate that pre‐malignant lesions obtained from non‐tobacco users do not exhibit pS3 mutations.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 458-463 |
| Number of pages | 6 |
| Journal | International Journal of Cancer |
| Volume | 60 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 8 1995 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
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A low incidence of p53 mutations in pre‐malignant lesions of the oral cavity from non‐tobacco users. / Lazarus, P.; Garewal, H. S.; Sciuuba, J.; Zwiebel, N.; Calcagnotto, A.; Fair, A.; Schaefer, S.; Richie, J. P.
In: International Journal of Cancer, Vol. 60, No. 4, 08.02.1995, p. 458-463.Research output: Contribution to journal › Article
TY - JOUR
T1 - A low incidence of p53 mutations in pre‐malignant lesions of the oral cavity from non‐tobacco users
AU - Lazarus, P.
AU - Garewal, H. S.
AU - Sciuuba, J.
AU - Zwiebel, N.
AU - Calcagnotto, A.
AU - Fair, A.
AU - Schaefer, S.
AU - Richie, J. P.
PY - 1995/2/8
Y1 - 1995/2/8
N2 - To determine the incidence of pS3 mutations in pre‐malignant lesions of the oral cavity from individuals without prior history of tobacco use, we have analyzed the conserved regions of the pS3 gene (exons 5‐9) in archival oral cavity lesion specimens obtained from patients with varied tobacco use histories, by polymerase chain reaction/single strand conformational polymorphism (PCR/SSCP) and DNA sequencing analysis. Twenty‐six lesions were analyzed from 14 patients, with multiple lesions obtained from 8 patients. Six of these patients used tobacco, (3 being cigarette smokers, 1 ex‐cigarette smoker, 1 moderate cigar smoker and 1 snuff chewer). The remaining 8 patients had no prior history of tobacco use. Thirteen of the pre‐malignant lesions exhibited severe dysplasia, 9 exhibited moderate dysplasia and 4 exhibited mild dysplasia. Four of the 26 lesions exhibited pS3 mutations, each being from a tobacco user. None of the 13 lesions from never‐tobacco users exhibited pS3 mutations. There was a significantly higher p53 mutation incidence in pre‐malignant lesions from tobacco users (including ex‐smokers) than in non‐tobacco users as well as in cigarette smokers plus snuff chewers than in non‐tobacco users. Two of the mutations were observed in lesions exhibiting severe dysplasia: I in a lesion exhibiting moderate dysplasia and I in a lesion exhibiting mild dysplasia. These data suggest that pS3 mutation may be a very early event in oral cavity tumor progression and demonstrate that pre‐malignant lesions obtained from non‐tobacco users do not exhibit pS3 mutations.
AB - To determine the incidence of pS3 mutations in pre‐malignant lesions of the oral cavity from individuals without prior history of tobacco use, we have analyzed the conserved regions of the pS3 gene (exons 5‐9) in archival oral cavity lesion specimens obtained from patients with varied tobacco use histories, by polymerase chain reaction/single strand conformational polymorphism (PCR/SSCP) and DNA sequencing analysis. Twenty‐six lesions were analyzed from 14 patients, with multiple lesions obtained from 8 patients. Six of these patients used tobacco, (3 being cigarette smokers, 1 ex‐cigarette smoker, 1 moderate cigar smoker and 1 snuff chewer). The remaining 8 patients had no prior history of tobacco use. Thirteen of the pre‐malignant lesions exhibited severe dysplasia, 9 exhibited moderate dysplasia and 4 exhibited mild dysplasia. Four of the 26 lesions exhibited pS3 mutations, each being from a tobacco user. None of the 13 lesions from never‐tobacco users exhibited pS3 mutations. There was a significantly higher p53 mutation incidence in pre‐malignant lesions from tobacco users (including ex‐smokers) than in non‐tobacco users as well as in cigarette smokers plus snuff chewers than in non‐tobacco users. Two of the mutations were observed in lesions exhibiting severe dysplasia: I in a lesion exhibiting moderate dysplasia and I in a lesion exhibiting mild dysplasia. These data suggest that pS3 mutation may be a very early event in oral cavity tumor progression and demonstrate that pre‐malignant lesions obtained from non‐tobacco users do not exhibit pS3 mutations.
UR - http://www.scopus.com/inward/record.url?scp=0028950119&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028950119&partnerID=8YFLogxK
U2 - 10.1002/ijc.2910600406
DO - 10.1002/ijc.2910600406
M3 - Article
C2 - 7829258
AN - SCOPUS:0028950119
VL - 60
SP - 458
EP - 463
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 4
ER -