A lower esophageal sphincter reflex involving substance P

J. C. Reynolds, Ann Ouyang, S. Cohen

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The purpose of this study was to determine the mechanism by which distal esophageal acidification increases lower esophageal sphincter (LES) pressure in the anesthetized cat. Intraluminal pressures and myoelectric activity were recorded using fixed, localized manometric catheters and serosal bipolar silver-silver chloride electrodes. The increase in LES pressure (27.1 ± 4.9 mmHg) and spike activity (133.8 ± 22.6 spikes/min) following distal esophageal acidification were greater than after saline (P < 0.001). These responses were abolished by either tetrodotoxin (intravenously) or intraluminal ethyl aminobenzoate. The responses were not antagonized by bilateral cervical vagotomy or by atropine, hexamethonium, phentolamine, propranolol, diphenydramine, cimetidine, cinanserin, naloxone, haloperidol, or proglumide. Tachyphylaxis to substance P abolished the LES pressure and spike responses to exogenous substance P and to distal esophageal acidification but had no effect on the LES responses to phenylephrine (25.0 μg/kg iv) or pentagastrin (0.5 μg/kg iv). The putative substance P antagonist [D-Pro 2 ,D-Trp 7,9 ]substance P was a partial antagonist and a weak agonist on the LES. Large doses of [D-Pro 2 ,D-TRP 7,9 ]substance P (200.0 μg/kg iv) gave a 61.3 ± 19.3% inhibition of the LES pressure response to acid (P < 0.05). Intravenous tetrodotoxin partially antagonized the LES response to substance P (10.0 μg/kg iv). These studies suggest that the increases in LES pressure and spike activity following distal esophageal acidification occur through a spike-associated enteric neural reflex that involves substance P as a neurotransmitter.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume9
Issue number4
StatePublished - Jan 1 1984

Fingerprint

Lower Esophageal Sphincter
Substance P
Reflex
Pressure
Tetrodotoxin
Cinanserin
Proglumide
Benzocaine
Tachyphylaxis
Pentagastrin
Hexamethonium
Phentolamine
Vagotomy
Cimetidine
Phenylephrine
Haloperidol
Naloxone
Atropine
Silver
Propranolol

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Cite this

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abstract = "The purpose of this study was to determine the mechanism by which distal esophageal acidification increases lower esophageal sphincter (LES) pressure in the anesthetized cat. Intraluminal pressures and myoelectric activity were recorded using fixed, localized manometric catheters and serosal bipolar silver-silver chloride electrodes. The increase in LES pressure (27.1 ± 4.9 mmHg) and spike activity (133.8 ± 22.6 spikes/min) following distal esophageal acidification were greater than after saline (P < 0.001). These responses were abolished by either tetrodotoxin (intravenously) or intraluminal ethyl aminobenzoate. The responses were not antagonized by bilateral cervical vagotomy or by atropine, hexamethonium, phentolamine, propranolol, diphenydramine, cimetidine, cinanserin, naloxone, haloperidol, or proglumide. Tachyphylaxis to substance P abolished the LES pressure and spike responses to exogenous substance P and to distal esophageal acidification but had no effect on the LES responses to phenylephrine (25.0 μg/kg iv) or pentagastrin (0.5 μg/kg iv). The putative substance P antagonist [D-Pro 2 ,D-Trp 7,9 ]substance P was a partial antagonist and a weak agonist on the LES. Large doses of [D-Pro 2 ,D-TRP 7,9 ]substance P (200.0 μg/kg iv) gave a 61.3 ± 19.3{\%} inhibition of the LES pressure response to acid (P < 0.05). Intravenous tetrodotoxin partially antagonized the LES response to substance P (10.0 μg/kg iv). These studies suggest that the increases in LES pressure and spike activity following distal esophageal acidification occur through a spike-associated enteric neural reflex that involves substance P as a neurotransmitter.",
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A lower esophageal sphincter reflex involving substance P. / Reynolds, J. C.; Ouyang, Ann; Cohen, S.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 9, No. 4, 01.01.1984.

Research output: Contribution to journalArticle

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