A Maize Inbred Exhibits Resistance Against Western Corn Rootwoorm, Diabrotica virgifera virgifera

Lina Castano-Duque, Kenneth W. Loades, John Frazier Tooker, Kathleen Marie Brown, W. Paul Williams, Dawn S. Luthe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Insect resistance against root herbivores like the western corn rootworm (WCR, Diabrotica virgifera virgifera) is not well understood in non-transgenic maize. We studied the responses of two American maize inbreds, Mp708 and Tx601, to WCR infestation using biomechanical, molecular, biochemical analyses, and laser ablation tomography. Previous studies performed on several inbreds indicated that these two maize genotypes differed in resistance to pests including fall armyworm (Spodoptera frugiperda) and WCR. Our data confirmed that Mp708 shows resistance against WCR, and demonstrates that the resistance mechanism is based in a multi-trait phenotype that includes increased resistance to cutting in nodal roots, stable root growth during insect infestation, constitutive and induced expression of known herbivore-defense genes, including ribosomal inhibitor protein 2 (rip2), terpene synthase 23 (tps23) and maize insect resistance cysteine protease-1 (mir1), as well high constitutive levels of jasmonic acid and production of (E)-β-caryophyllene. In contrast, Tx601 is susceptible to WCR. These findings will facilitate the use of Mp708 as a model to explore the wide variety of mechanisms and traits involved in plant defense responses and resistance to herbivory by insects with several different feeding habits.

Original languageEnglish (US)
Pages (from-to)1109-1123
Number of pages15
JournalJournal of Chemical Ecology
Volume43
Issue number11-12
DOIs
StatePublished - Dec 1 2017

Fingerprint

Diabrotica virgifera virgifera
Cysteine Proteases
Laser ablation
Zea mays
Tomography
Genes
maize
Herbivory
Insects
herbivores
corn
Spodoptera frugiperda
insect
insects
Proteins
herbivore
cysteine proteinases
tomography
jasmonic acid
Spodoptera

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Castano-Duque, Lina ; Loades, Kenneth W. ; Tooker, John Frazier ; Brown, Kathleen Marie ; Paul Williams, W. ; Luthe, Dawn S. / A Maize Inbred Exhibits Resistance Against Western Corn Rootwoorm, Diabrotica virgifera virgifera. In: Journal of Chemical Ecology. 2017 ; Vol. 43, No. 11-12. pp. 1109-1123.
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A Maize Inbred Exhibits Resistance Against Western Corn Rootwoorm, Diabrotica virgifera virgifera. / Castano-Duque, Lina; Loades, Kenneth W.; Tooker, John Frazier; Brown, Kathleen Marie; Paul Williams, W.; Luthe, Dawn S.

In: Journal of Chemical Ecology, Vol. 43, No. 11-12, 01.12.2017, p. 1109-1123.

Research output: Contribution to journalArticle

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