A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate

Rahul Bhosale; Jitender Giri; Bipin K. Pandey; Ricardo F.H. Giehl; Anja Hartmann; Richard Traini; Jekaterina Truskina; Nicola Leftley; Meredith Hanlon; Kamal Swarup; Afaf Rashed; Ute Voß; Jose Alonso; Anna Stepanova; Jeonga Yun; Karin Ljung; Kathleen M. Brown; Jonathan P. Lynch; Liam Dolan; Teva Vernoux; Anthony Bishopp; Darren Wells; Nicolaus Von Wirén; Malcolm J. Bennett; Ranjan Swarup

doi:10.1038/s41467-018-03851-3

A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate

Rahul Bhosale, Jitender Giri, Bipin K. Pandey, Ricardo F.H. Giehl, Anja Hartmann, Richard Traini, Jekaterina Truskina, Nicola Leftley, Meredith Hanlon, Kamal Swarup, Afaf Rashed, Ute Voß, Jose Alonso, Anna Stepanova, Jeonga Yun, Karin Ljung, Kathleen M. Brown, Jonathan P. Lynch, Liam Dolan, Teva VernouxAnthony Bishopp, Darren Wells, Nicolaus Von Wirén, Malcolm J. Bennett, Ranjan Swarup

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Abstract

Phosphate (P) is an essential macronutrient for plant growth. Roots employ adaptive mechanisms to forage for P in soil. Root hair elongation is particularly important since P is immobile. Here we report that auxin plays a critical role promoting root hair growth in Arabidopsis in response to low external P. Mutants disrupting auxin synthesis (taa1) and transport (aux1) attenuate the low P root hair response. Conversely, targeting AUX1 expression in lateral root cap and epidermal cells rescues this low P response in aux1. Hence auxin transport from the root apex to differentiation zone promotes auxin-dependent hair response to low P. Low external P results in induction of root hair expressed auxin-inducible transcription factors ARF19, RSL2, and RSL4. Mutants lacking these genes disrupt the low P root hair response. We conclude auxin synthesis, transport and response pathway components play critical roles regulating this low P root adaptive response.

Original language	English (US)
Article number	1409
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-03851-3
State	Published - Dec 1 2018

All Science Journal Classification (ASJC) codes

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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Bhosale, R., Giri, J., Pandey, B. K., Giehl, R. F. H., Hartmann, A., Traini, R., Truskina, J., Leftley, N., Hanlon, M., Swarup, K., Rashed, A., Voß, U., Alonso, J., Stepanova, A., Yun, J., Ljung, K., Brown, K. M., Lynch, J. P., Dolan, L., ... Swarup, R. (2018). A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate. Nature communications, 9(1), [1409]. https://doi.org/10.1038/s41467-018-03851-3

Bhosale, Rahul ; Giri, Jitender ; Pandey, Bipin K. ; Giehl, Ricardo F.H. ; Hartmann, Anja ; Traini, Richard ; Truskina, Jekaterina ; Leftley, Nicola ; Hanlon, Meredith ; Swarup, Kamal ; Rashed, Afaf ; Voß, Ute ; Alonso, Jose ; Stepanova, Anna ; Yun, Jeonga ; Ljung, Karin ; Brown, Kathleen M. ; Lynch, Jonathan P. ; Dolan, Liam ; Vernoux, Teva ; Bishopp, Anthony ; Wells, Darren ; Von Wirén, Nicolaus ; Bennett, Malcolm J. ; Swarup, Ranjan. / A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate. In: Nature communications. 2018 ; Vol. 9, No. 1.

@article{a93ffdbf81604b119599a2ec73e6a37d,

title = "A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate",

abstract = "Phosphate (P) is an essential macronutrient for plant growth. Roots employ adaptive mechanisms to forage for P in soil. Root hair elongation is particularly important since P is immobile. Here we report that auxin plays a critical role promoting root hair growth in Arabidopsis in response to low external P. Mutants disrupting auxin synthesis (taa1) and transport (aux1) attenuate the low P root hair response. Conversely, targeting AUX1 expression in lateral root cap and epidermal cells rescues this low P response in aux1. Hence auxin transport from the root apex to differentiation zone promotes auxin-dependent hair response to low P. Low external P results in induction of root hair expressed auxin-inducible transcription factors ARF19, RSL2, and RSL4. Mutants lacking these genes disrupt the low P root hair response. We conclude auxin synthesis, transport and response pathway components play critical roles regulating this low P root adaptive response.",

author = "Rahul Bhosale and Jitender Giri and Pandey, {Bipin K.} and Giehl, {Ricardo F.H.} and Anja Hartmann and Richard Traini and Jekaterina Truskina and Nicola Leftley and Meredith Hanlon and Kamal Swarup and Afaf Rashed and Ute Vo{\ss} and Jose Alonso and Anna Stepanova and Jeonga Yun and Karin Ljung and Brown, {Kathleen M.} and Lynch, {Jonathan P.} and Liam Dolan and Teva Vernoux and Anthony Bishopp and Darren Wells and {Von Wir{\'e}n}, Nicolaus and Bennett, {Malcolm J.} and Ranjan Swarup",

note = "Funding Information: This work was supported by the awards from the Biotechnology and Biological Sciences Research Council [grant numbers BB/G023972/1, BB/R013748/1, BB/L026848/1, BB/M018431/1, BB/PO16855/1, BB/M001806/1, BB/P010520/1]; the European Research Council FUTUREROOTS Advanced Investigator grant [grant number 294729]; Leverhulme Trust [grant number RPG-2016-409]; Royal Society [grant number WM130021, NA140281]; Newton International Fellowship (NF140287) and British Council Newton Bhabha (228144076). This work was also supported by funds from the University of Nottingham Future Food Beacon of Excellence Nottingham Research and PhD+ fellowship schemes; the Interuniversity Attraction Poles Program initiated by the Belgian Science Policy Office [P7/29]; the Swedish Governmental Agency for Innovation Systems (VINNOVA), and the Swedish Research Council (V.R.). NSF-MCB1158181 and a joint studentship between the University of Nottingham and Institut National de la Recherche Agronomique (INRA). We also thank Roger Granbom (Swedish University of Agricultural Sciences) for skilful technical assistance. Publisher Copyright: {\textcopyright} 2018 The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41467-018-03851-3",

language = "English (US)",

volume = "9",

journal = "Nature Communications",

issn = "2041-1723",

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Bhosale, R, Giri, J, Pandey, BK, Giehl, RFH, Hartmann, A, Traini, R, Truskina, J, Leftley, N, Hanlon, M, Swarup, K, Rashed, A, Voß, U, Alonso, J, Stepanova, A, Yun, J, Ljung, K, Brown, KM, Lynch, JP, Dolan, L, Vernoux, T, Bishopp, A, Wells, D, Von Wirén, N, Bennett, MJ & Swarup, R 2018, 'A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate', Nature communications, vol. 9, no. 1, 1409. https://doi.org/10.1038/s41467-018-03851-3

A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate. / Bhosale, Rahul; Giri, Jitender; Pandey, Bipin K.; Giehl, Ricardo F.H.; Hartmann, Anja; Traini, Richard; Truskina, Jekaterina; Leftley, Nicola; Hanlon, Meredith; Swarup, Kamal; Rashed, Afaf; Voß, Ute; Alonso, Jose; Stepanova, Anna; Yun, Jeonga; Ljung, Karin; Brown, Kathleen M.; Lynch, Jonathan P.; Dolan, Liam; Vernoux, Teva; Bishopp, Anthony; Wells, Darren; Von Wirén, Nicolaus; Bennett, Malcolm J.; Swarup, Ranjan.

In: Nature communications, Vol. 9, No. 1, 1409, 01.12.2018.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate

AU - Bhosale, Rahul

AU - Giri, Jitender

AU - Pandey, Bipin K.

AU - Giehl, Ricardo F.H.

AU - Hartmann, Anja

AU - Traini, Richard

AU - Truskina, Jekaterina

AU - Leftley, Nicola

AU - Hanlon, Meredith

AU - Swarup, Kamal

AU - Rashed, Afaf

AU - Voß, Ute

AU - Alonso, Jose

AU - Stepanova, Anna

AU - Yun, Jeonga

AU - Ljung, Karin

AU - Brown, Kathleen M.

AU - Lynch, Jonathan P.

AU - Dolan, Liam

AU - Vernoux, Teva

AU - Bishopp, Anthony

AU - Wells, Darren

AU - Von Wirén, Nicolaus

AU - Bennett, Malcolm J.

AU - Swarup, Ranjan

N1 - Funding Information: This work was supported by the awards from the Biotechnology and Biological Sciences Research Council [grant numbers BB/G023972/1, BB/R013748/1, BB/L026848/1, BB/M018431/1, BB/PO16855/1, BB/M001806/1, BB/P010520/1]; the European Research Council FUTUREROOTS Advanced Investigator grant [grant number 294729]; Leverhulme Trust [grant number RPG-2016-409]; Royal Society [grant number WM130021, NA140281]; Newton International Fellowship (NF140287) and British Council Newton Bhabha (228144076). This work was also supported by funds from the University of Nottingham Future Food Beacon of Excellence Nottingham Research and PhD+ fellowship schemes; the Interuniversity Attraction Poles Program initiated by the Belgian Science Policy Office [P7/29]; the Swedish Governmental Agency for Innovation Systems (VINNOVA), and the Swedish Research Council (V.R.). NSF-MCB1158181 and a joint studentship between the University of Nottingham and Institut National de la Recherche Agronomique (INRA). We also thank Roger Granbom (Swedish University of Agricultural Sciences) for skilful technical assistance. Publisher Copyright: © 2018 The Author(s).

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Phosphate (P) is an essential macronutrient for plant growth. Roots employ adaptive mechanisms to forage for P in soil. Root hair elongation is particularly important since P is immobile. Here we report that auxin plays a critical role promoting root hair growth in Arabidopsis in response to low external P. Mutants disrupting auxin synthesis (taa1) and transport (aux1) attenuate the low P root hair response. Conversely, targeting AUX1 expression in lateral root cap and epidermal cells rescues this low P response in aux1. Hence auxin transport from the root apex to differentiation zone promotes auxin-dependent hair response to low P. Low external P results in induction of root hair expressed auxin-inducible transcription factors ARF19, RSL2, and RSL4. Mutants lacking these genes disrupt the low P root hair response. We conclude auxin synthesis, transport and response pathway components play critical roles regulating this low P root adaptive response.

AB - Phosphate (P) is an essential macronutrient for plant growth. Roots employ adaptive mechanisms to forage for P in soil. Root hair elongation is particularly important since P is immobile. Here we report that auxin plays a critical role promoting root hair growth in Arabidopsis in response to low external P. Mutants disrupting auxin synthesis (taa1) and transport (aux1) attenuate the low P root hair response. Conversely, targeting AUX1 expression in lateral root cap and epidermal cells rescues this low P response in aux1. Hence auxin transport from the root apex to differentiation zone promotes auxin-dependent hair response to low P. Low external P results in induction of root hair expressed auxin-inducible transcription factors ARF19, RSL2, and RSL4. Mutants lacking these genes disrupt the low P root hair response. We conclude auxin synthesis, transport and response pathway components play critical roles regulating this low P root adaptive response.

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U2 - 10.1038/s41467-018-03851-3

DO - 10.1038/s41467-018-03851-3

M3 - Article

C2 - 29651114

AN - SCOPUS:85045517504

VL - 9

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 1409

ER -

A mechanistic framework for auxin dependent Arabidopsis root hair elongation to low external phosphate

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