A mechanoregulation approach for probing substrate elasticities and geometric constraints on differentiation of human neuroblastoma

K. H. Nam, E. Mfoumou, P. K. Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cellular differentiations are regulated by various mechanical and physical factors in their local microenvironment, such as cell-substrate interactions and geometric confinements. Understanding the systematic mechanisms of these factors in cell mechanoregulation is critical for fundamental cell biology and development of novel approaches in tissue engineering and regenerative medicine. Here, we investigate the differentiation of human neuroblastoma cells on various substrates with different elastic moduli controlled by the crosslinker concentration of PDMS and with different geometric constraints created by plasma lithographic surface modification. This mechanoregulation approach allows systematic investigation of the effects of the interrelated mechanical factors, i.e., geometric confinement and substrate elasticity, on cellular differentiation and other biological processes.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1008-1010
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Elasticity
Substrates
Cytology
Tissue engineering
Surface treatment
Elastic moduli
Plasmas
Regenerative Medicine

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Nam, K. H., Mfoumou, E., & Wong, P. K. (2011). A mechanoregulation approach for probing substrate elasticities and geometric constraints on differentiation of human neuroblastoma. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1008-1010). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).
Nam, K. H. ; Mfoumou, E. ; Wong, P. K. / A mechanoregulation approach for probing substrate elasticities and geometric constraints on differentiation of human neuroblastoma. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1008-1010 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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abstract = "Cellular differentiations are regulated by various mechanical and physical factors in their local microenvironment, such as cell-substrate interactions and geometric confinements. Understanding the systematic mechanisms of these factors in cell mechanoregulation is critical for fundamental cell biology and development of novel approaches in tissue engineering and regenerative medicine. Here, we investigate the differentiation of human neuroblastoma cells on various substrates with different elastic moduli controlled by the crosslinker concentration of PDMS and with different geometric constraints created by plasma lithographic surface modification. This mechanoregulation approach allows systematic investigation of the effects of the interrelated mechanical factors, i.e., geometric confinement and substrate elasticity, on cellular differentiation and other biological processes.",
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Nam, KH, Mfoumou, E & Wong, PK 2011, A mechanoregulation approach for probing substrate elasticities and geometric constraints on differentiation of human neuroblastoma. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 2, pp. 1008-1010, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.

A mechanoregulation approach for probing substrate elasticities and geometric constraints on differentiation of human neuroblastoma. / Nam, K. H.; Mfoumou, E.; Wong, P. K.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1008-1010 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Nam KH, Mfoumou E, Wong PK. A mechanoregulation approach for probing substrate elasticities and geometric constraints on differentiation of human neuroblastoma. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1008-1010. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).