A membrane-delimited pathway of G-protein regulation of the guard-cell inward K+ channel

Wei Hua Wu, Sarah M. Assmann

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78 Citations (Scopus)

Abstract

GTP-binding protein (G-protein) regulation of inward rectifying K+ channels in the plasma membrane of Vicia (Vicia faba L.) guard cells has previously been demonstrated at the whole-cell level. However, whether a cytosolic signal transduction chain is required for G-protein regulation of K+ channels in Vicia guard cells, or in any plant cell type, remains unknown. In the present study, we assayed effects of several G-protein regulators on inward K+ channels in isolated inside-out membrane patches from Vicia guard cell protoplasts. Guanosine 5'-[γ-thio]triphosphate, a nonhydrolyzable GTP analog that locks G proteins into their activated state, decreased the open state probability (P(o)) of single inward K+ channels. This decrease in P(o) was accompanied by an increase in one of the closed time constants of the K+ channel. Guanosine 5'-[β-thio]diphosphate, a GDP analog that locks G proteins into their inactivated state, slightly increased the P(o) of the inward K+ channel and shortened the closed time constants. Pertussis toxin and cholera toxin, which ADP-ribosylate G proteins at different sites, decreased the P(o) of the inward K+ channel. Our data indicate that G proteins can act via a membrane-delimited pathway to regulate inward K+ channels in the guard-cell plasma membrane.

Original languageEnglish (US)
Pages (from-to)6310-6314
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number14
DOIs
StatePublished - Jul 5 1994

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GTP-Binding Proteins
Vicia
Membranes
Guanosine
Cell Membrane
GTP-Binding Protein Regulators
Vicia faba
Protoplasts
Diphosphates
Cholera Toxin
Pertussis Toxin
Plant Cells
Guanosine Triphosphate
Adenosine Diphosphate
Signal Transduction

All Science Journal Classification (ASJC) codes

  • General

Cite this

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abstract = "GTP-binding protein (G-protein) regulation of inward rectifying K+ channels in the plasma membrane of Vicia (Vicia faba L.) guard cells has previously been demonstrated at the whole-cell level. However, whether a cytosolic signal transduction chain is required for G-protein regulation of K+ channels in Vicia guard cells, or in any plant cell type, remains unknown. In the present study, we assayed effects of several G-protein regulators on inward K+ channels in isolated inside-out membrane patches from Vicia guard cell protoplasts. Guanosine 5'-[γ-thio]triphosphate, a nonhydrolyzable GTP analog that locks G proteins into their activated state, decreased the open state probability (P(o)) of single inward K+ channels. This decrease in P(o) was accompanied by an increase in one of the closed time constants of the K+ channel. Guanosine 5'-[β-thio]diphosphate, a GDP analog that locks G proteins into their inactivated state, slightly increased the P(o) of the inward K+ channel and shortened the closed time constants. Pertussis toxin and cholera toxin, which ADP-ribosylate G proteins at different sites, decreased the P(o) of the inward K+ channel. Our data indicate that G proteins can act via a membrane-delimited pathway to regulate inward K+ channels in the guard-cell plasma membrane.",
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T1 - A membrane-delimited pathway of G-protein regulation of the guard-cell inward K+ channel

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AU - Assmann, Sarah M.

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N2 - GTP-binding protein (G-protein) regulation of inward rectifying K+ channels in the plasma membrane of Vicia (Vicia faba L.) guard cells has previously been demonstrated at the whole-cell level. However, whether a cytosolic signal transduction chain is required for G-protein regulation of K+ channels in Vicia guard cells, or in any plant cell type, remains unknown. In the present study, we assayed effects of several G-protein regulators on inward K+ channels in isolated inside-out membrane patches from Vicia guard cell protoplasts. Guanosine 5'-[γ-thio]triphosphate, a nonhydrolyzable GTP analog that locks G proteins into their activated state, decreased the open state probability (P(o)) of single inward K+ channels. This decrease in P(o) was accompanied by an increase in one of the closed time constants of the K+ channel. Guanosine 5'-[β-thio]diphosphate, a GDP analog that locks G proteins into their inactivated state, slightly increased the P(o) of the inward K+ channel and shortened the closed time constants. Pertussis toxin and cholera toxin, which ADP-ribosylate G proteins at different sites, decreased the P(o) of the inward K+ channel. Our data indicate that G proteins can act via a membrane-delimited pathway to regulate inward K+ channels in the guard-cell plasma membrane.

AB - GTP-binding protein (G-protein) regulation of inward rectifying K+ channels in the plasma membrane of Vicia (Vicia faba L.) guard cells has previously been demonstrated at the whole-cell level. However, whether a cytosolic signal transduction chain is required for G-protein regulation of K+ channels in Vicia guard cells, or in any plant cell type, remains unknown. In the present study, we assayed effects of several G-protein regulators on inward K+ channels in isolated inside-out membrane patches from Vicia guard cell protoplasts. Guanosine 5'-[γ-thio]triphosphate, a nonhydrolyzable GTP analog that locks G proteins into their activated state, decreased the open state probability (P(o)) of single inward K+ channels. This decrease in P(o) was accompanied by an increase in one of the closed time constants of the K+ channel. Guanosine 5'-[β-thio]diphosphate, a GDP analog that locks G proteins into their inactivated state, slightly increased the P(o) of the inward K+ channel and shortened the closed time constants. Pertussis toxin and cholera toxin, which ADP-ribosylate G proteins at different sites, decreased the P(o) of the inward K+ channel. Our data indicate that G proteins can act via a membrane-delimited pathway to regulate inward K+ channels in the guard-cell plasma membrane.

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