A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)

Baneshwar Singh, Tawni L. Crippen, Longyu Zheng, Andrew T. Fields, Ziniu Yu, Qun Ma, Thomas Keith Wood, Scot E. Dowd, Micah Flores, Jeffery K. Tomberlin, Aaron M. Tarone

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods.

Original languageEnglish (US)
Pages (from-to)869-883
Number of pages15
JournalApplied Microbiology and Biotechnology
Volume99
Issue number2
DOIs
StatePublished - Jan 1 2015

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Metagenomics
Diptera
Salivary Glands
Bacteria
Morganella
Lactobacillus
Debridement
Transmission Electron Microscopy
Larva
Providencia
Lactococcus
Bacteroidetes
Proteobacteria
Proteus
Disease Management
Ribosomal DNA
Gram-Negative Bacteria
Eggs
Economics
Light

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Singh, Baneshwar ; Crippen, Tawni L. ; Zheng, Longyu ; Fields, Andrew T. ; Yu, Ziniu ; Ma, Qun ; Wood, Thomas Keith ; Dowd, Scot E. ; Flores, Micah ; Tomberlin, Jeffery K. ; Tarone, Aaron M. / A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera : Calliphoridae). In: Applied Microbiology and Biotechnology. 2015 ; Vol. 99, No. 2. pp. 869-883.
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abstract = "Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 {\%} genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods.",
author = "Baneshwar Singh and Crippen, {Tawni L.} and Longyu Zheng and Fields, {Andrew T.} and Ziniu Yu and Qun Ma and Wood, {Thomas Keith} and Dowd, {Scot E.} and Micah Flores and Tomberlin, {Jeffery K.} and Tarone, {Aaron M.}",
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Singh, B, Crippen, TL, Zheng, L, Fields, AT, Yu, Z, Ma, Q, Wood, TK, Dowd, SE, Flores, M, Tomberlin, JK & Tarone, AM 2015, 'A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera: Calliphoridae)', Applied Microbiology and Biotechnology, vol. 99, no. 2, pp. 869-883. https://doi.org/10.1007/s00253-014-6115-7

A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera : Calliphoridae). / Singh, Baneshwar; Crippen, Tawni L.; Zheng, Longyu; Fields, Andrew T.; Yu, Ziniu; Ma, Qun; Wood, Thomas Keith; Dowd, Scot E.; Flores, Micah; Tomberlin, Jeffery K.; Tarone, Aaron M.

In: Applied Microbiology and Biotechnology, Vol. 99, No. 2, 01.01.2015, p. 869-883.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A metagenomic assessment of the bacteria associated with Lucilia sericata and Lucilia cuprina (Diptera

T2 - Calliphoridae)

AU - Singh, Baneshwar

AU - Crippen, Tawni L.

AU - Zheng, Longyu

AU - Fields, Andrew T.

AU - Yu, Ziniu

AU - Ma, Qun

AU - Wood, Thomas Keith

AU - Dowd, Scot E.

AU - Flores, Micah

AU - Tomberlin, Jeffery K.

AU - Tarone, Aaron M.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods.

AB - Lucilia Robineau-Desvoidy (Diptera: Calliphoridae) is a blow fly genus of forensic, medical, veterinary, and agricultural importance. This genus is also famous because of its beneficial uses in maggot debridement therapy (MDT). Although the genus is of considerable economic importance, our knowledge about microbes associated with these flies and how these bacteria are horizontally and trans-generationally transmitted is limited. In this study, we characterized bacteria associated with different life stages of Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) and in the salivary gland of L. sericata by using 16S rDNA 454 pyrosequencing. Bacteria associated with the salivary gland of L. sericata were also characterized using light and transmission electron microscopy (TEM). Results from this study suggest that the majority of bacteria associated with these flies belong to phyla Proteobacteria, Firmicutes, and Bacteroidetes, and most bacteria are maintained intragenerationally, with a considerable degree of turnover from generation to generation. In both species, second-generation eggs exhibited the highest bacterial phylum diversity (20 % genetic distance) than other life stages. The Lucilia sister species shared the majority of their classified genera. Of the shared bacterial genera, Providencia, Ignatzschineria, Lactobacillus, Lactococcus, Vagococcus, Morganella, and Myroides were present at relatively high abundances. Lactobacillus, Proteus, Diaphorobacter, and Morganella were the dominant bacterial genera associated with a survey of the salivary gland of L. sericata. TEM analysis showed a sparse distribution of both Gram-positive and Gram-negative bacteria in the salivary gland of L. sericata. There was more evidence for horizontal transmission of bacteria than there was for trans-generational inheritance. Several pathogenic genera were either amplified or reduced by the larval feeding on decomposing liver as a resource. Overall, this study provides information on bacterial communities associated with different life stages of Lucilia and their horizontal and trans-generational transmission, which may help in the development of better vector-borne disease management and MDT methods.

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