A micro device for separation of erythrocytes and leukocytes in human blood

Siyang Zheng, Yu Chong Tai, Harvey Kasdan

Research output: Chapter in Book/Report/Conference proceedingConference contribution

15 Citations (Scopus)

Abstract

We report a MEMS device for continuous and binary separation of leukocytes (white blood cells, WBCs) and erythrocytes (red blood cells, RBCs) by their sizes. The system consists of PDMS cylindrical pillars inside fluidic chamber on glass slides. A 420 μm lateral separation was achieved for 5 μm and 10 μm beads. The critical particle size for separation was successful predicted by simulation and experimentally determined by calibration with polystyrene beads. The separation function (lateral displacement versus particle size) was also measured experimentally. Diluted whole blood and blood fraction of concentrated leukocytes were tested with the devices with erythrocyte to leukocyte ratio in agreement with blood count results. Experiments with fluorescent labeled leukocytes confirm the separation of leukocytes from erythrocytes and measured the separation efficiency.

Original languageEnglish (US)
Title of host publicationProceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
Pages1024-1027
Number of pages4
StatePublished - Dec 1 2005
Event2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 - Shanghai, China
Duration: Sep 1 2005Sep 4 2005

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume7 VOLS
ISSN (Print)0589-1019

Other

Other2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
CountryChina
CityShanghai
Period9/1/059/4/05

Fingerprint

Leukocytes
Blood
Erythrocytes
Equipment and Supplies
Particle Size
Particle size
Cells
Micro-Electrical-Mechanical Systems
Polystyrenes
Fluidics
Cell Size
Calibration
MEMS
Glass
Experiments

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

Zheng, S., Tai, Y. C., & Kasdan, H. (2005). A micro device for separation of erythrocytes and leukocytes in human blood. In Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 (pp. 1024-1027). [1616592] (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 7 VOLS).
Zheng, Siyang ; Tai, Yu Chong ; Kasdan, Harvey. / A micro device for separation of erythrocytes and leukocytes in human blood. Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005. 2005. pp. 1024-1027 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).
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Zheng, S, Tai, YC & Kasdan, H 2005, A micro device for separation of erythrocytes and leukocytes in human blood. in Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005., 1616592, Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, vol. 7 VOLS, pp. 1024-1027, 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005, Shanghai, China, 9/1/05.

A micro device for separation of erythrocytes and leukocytes in human blood. / Zheng, Siyang; Tai, Yu Chong; Kasdan, Harvey.

Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005. 2005. p. 1024-1027 1616592 (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings; Vol. 7 VOLS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Zheng S, Tai YC, Kasdan H. A micro device for separation of erythrocytes and leukocytes in human blood. In Proceedings of the 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005. 2005. p. 1024-1027. 1616592. (Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings).