A microfiltration device for urogenital schistosomiasis diagnostics

Yuan Xiao, Yi Lu, Michael Hsieh, Joseph Liao, Pak Kin Wong

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Schistosomiasis is a parasitic disease affecting over 200 million people worldwide. This study reports the design and development of a microfiltration device for isolating schistosome eggs in urine for rapid diagnostics of urogenital schistosomiasis. The design of the device comprises a linear array of microfluidic traps to immobilize and separate schistosome eggs. Sequential loading of individual eggs is achieved autonomously by flow resistance, which facilitates observation and enumeration of samples with low-abundance targets. Computational fluid dynamics modeling and experimental characterization are performed to optimize the trapping performance. By optimizing the capture strategy, the trapping efficiency could be achieved at 100% with 300 μl/min and 83% with 3000 μl/min, and the filtration procedure could be finished within 10 min. The trapped eggs can be either recovered for downstream analysis or preserved in situ for whole-mount staining. On-chip phenotyping using confocal laser fluorescence microscopy identifies the microstructure of the trapped schistosome eggs. The device provides a novel microfluidic approach for trapping, counting and on-chip fluorescence characterization of urinal Schistosoma haematobium eggs for clinical and investigative application.

Original languageEnglish (US)
Article numbere0154640
JournalPloS one
Volume11
Issue number4
DOIs
StatePublished - Apr 1 2016

Fingerprint

Schistosomiasis haematobia
microfiltration
Microfiltration
Schistosoma
Microfluidics
Eggs
trapping
Equipment and Supplies
Fluorescence microscopy
Schistosoma haematobium
flow resistance
Computational fluid dynamics
schistosomiasis
Fluorescence
parasitoses
fluorescence microscopy
Confocal Microscopy
microstructure
Microstructure
lasers

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Xiao, Yuan ; Lu, Yi ; Hsieh, Michael ; Liao, Joseph ; Wong, Pak Kin. / A microfiltration device for urogenital schistosomiasis diagnostics. In: PloS one. 2016 ; Vol. 11, No. 4.
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A microfiltration device for urogenital schistosomiasis diagnostics. / Xiao, Yuan; Lu, Yi; Hsieh, Michael; Liao, Joseph; Wong, Pak Kin.

In: PloS one, Vol. 11, No. 4, e0154640, 01.04.2016.

Research output: Contribution to journalArticle

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